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PARP Mouse Monoclonal Antibody (clone 123)
Poly(ADP-ribose) polymerase (PARP) is a 116 kDa nuclear protein which facilitates DNA repair by binding to DNA breaks and attracting DNA repair proteins to the site of damage. PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation.
Isotype
Mouse IgG1
Clone/PAD
123
Immunogen
Recombinant protein derived from the C-terminal region of human PARP protein.
Specificity
This antibody is specific for human PARP (PARP-1, NAD(+) ADP-ribosyltransferase 1, poly[ADP-ribose] synthetase 1) protein (accession # NP_001609, P09874), which is 96% homologous with Rhesus monkey and canine, 95% with rat and mouse and 94% with horse. On Western blots of human HeLa and Jurkat cell lysates, it identifies the target band at ~116 kDa.
Reactivity
Reactivity has been confirmed with human HeLa and Jurkat cell lysates using Western blotting. The reactivity has been also confirmed with human Jurkat cells using immunoprecipitation and immunofluorescence. Based on amino acid sequence homology, reactivity with Rhesus monkey, canine, rat and mouse as well as horse is also expected.
Applications
Immunofluorescence, Immunoprecipitation, Western Blot
(IF, IP, WB)
Reactivity: Rat,
Equine,
Mouse,
Canine,
Human,
Rhesus Monkey
Validated Application: Immunoprecipitation,
Western Blot,
Immunofluorescence
Host: Mouse
Clonality: Monoclonal
Clone ID: 123
Target Gene: PARP9
Gene ID: 142
Label or Dye: Unconjugated
Product Size: 100 µg
Shipping Condition: Wet Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Store in freezer (-5 to -30°C). |