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pcDNA™6.2/V5-PL-DEST Mammalian Expression Vector
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA6.2/V5-pL-DEST vector offers the following key features:
•Promoterless version of the pcDNA™6.2⁄V5-DEST vector (cat. no. 12489027)
•attR sites for Gateway® cloning
•Compatible with MultiSite Gateway® Pro kits (e.g. cat. no. 12537100)
•C-terminal V5 tag for easy detection
•Blasticidin resistance gene for efficient stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
Gateway® Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.
Additional materials required, available separately: Gateway® entry clone, appropriate Gateway® LR Clonase® enzyme mix, and reaction buffer
Promoter: None (Promoterless)
Key Function: Clone your own Promoter,
Multi-Gene Expression
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Transfection
Cloning Method: Gateway®
Vector Type: MultiSite Gateway Vectors,
pDEST,
pcDNA
Expression: Mammalian
Product Size: 6 µg
Protein Tag Position (to your gene): C-terminal
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
All destination vectors are provided lyophilized and supercoiled. |