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MultiSite Gateway® Pro 4.0, for cloning four DNA fragments into a Gateway® destination vector
MultiSite Gateway® Pro Technology enables you to efficiently and conveniently assemble multiple DNA fragments in the desired order and orientation into a Gateway® Expression vector. Using specifically designed att sites for recombinational cloning, you can clone two, three, or four DNA fragments into any Gateway® Destination vector containing attR1 and attR2 sites. The resulting expression clone is ready for downstream expression and analysis applications. The new MultiSite Gateway® Pro Technology allows you to:
• Clone multiple DNA fragments into one vector without using restriction enzymes or ligases
• Take full advantage of the wide selection of Gateway® Destination vectors (available from Invitrogen) or create your own
• Replace multiple plasmid transfections with a single vector carrying all DNA elements of interest
MultiSite Gateway® Pro Kits are available for cloning two, three, or four DNA fragments and for flexible and customized configuration of DNA fragments into a Gateway® Destination vector. All of the kits include internal positive control entry clones for troubleshooting potential entry clone issues.
Promoter: None (Promoterless)
Vector Type: MultiSite Gateway Vectors
Key Function: Clone your own Promoter,
Multi-Gene Expression
Product Size: 20 reactions
Cloning Method: Gateway®
Number of Fragments: 4 Fragments
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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