Plasmid pCMV•SPORT-βgal Plasmid pCMV·SPORT-bgal is used as a positive control for monitoring expression in eukaryotic cells. The plasmid contains the reporter gene b-galactosidase (b-gal) from E. coli cloned as a Not I fragment into plasmid pCMV·SPORT1. The plasmid also contains a CMV promoter upstream of the b-gal gene, followed by the SV40 t-intron and polyadenylation signal. The b-lactamase gene allows selection for ampicillin resistance in E. coli. Promoter: CMV Key Function: Constitutive Expression, Reporter Assays Protein Tag or Fusion: Untagged Reporter: Beta-Gal (lacZ) Delivery Method: Transfection Selection Agent (Eukaryotic): None Vector Type: pCMV Product Size: 25 µg Shipping Condition: Approved for shipment on Wet or Dry Ice Constitutive or Inducible System: Constitutive Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures. Store at +4°C. Store at -20°C long term.