Bac-to-Bac® HT Vector Kit Bac-to-Bac®HT Vector Kit 设计用于在大肠杆菌 (E. coli) 中生成杆状病毒载体后，在 Sf9、Sf21 或 High Five™ 细胞中表达和纯化带组氨酸标签的重组蛋白。 pFastBac™HT 载体具有以下特点： ■用于表达蛋白质的多角体强启动子 ■用于简化克隆的三个读码框 ■用于轻松纯化重组融合蛋白的 N-端 6xHis 标签 ■用于在蛋白质纯化后去除组氨酸标签的 TEV 蛋白酶切割位点 Cleavage: TEV Protease Recognition Site Promoter: Polyhedrin Vector Type: pFastBac Product Size: 1 kit Cloning Method: Restriction Enzyme ⁄ MCS Protein Tag or Fusion: His Tag (6x) Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures. The Bac-to-Bac® HT Vector Kit includes 10 µg of pFastBac™ HT vector and pFastBac HT-CAT control vector. Store the vectors at +4°C. All components are guaranteed stable for 6 months when properly stored