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Dynabeads® Protein G for Immunoprecipitation
Dynabeads® Protein G are uniform, 2.8 µm superparamagnetic beads with recombinant Protein G (approximately 17 kDa) covalently coupled to the surface. Dynabeads® Protein G provide a superior alternative to Sepharose® or agarose slurry for immunoprecipitation (IP):
• Simple, short ~30 minute procedure
• Significantly reduces background caused by nonspecific binding
• No columns, centrifugations, or time-consuming pre-treatment of your samples
• Gentle separation technology preserves native protein conformations and large-protein complexes
Magnetic bead-based separation is rapid and easy to perform
Antibody is added to the Dynabeads® Protein G. During a short incubation, the antibody binds to the Dynabeads® via their Fc-region. The tube is then placed on a magnet, where the beads migrate to the side of the tube facing the magnet. The supernatant is then removed by aspiration. The bead-bound antibody may now be used for a variety of downstream applications including immunoprecipitation,
Ligand Type: Protein G
Form: Beads in Suspension
Shelf Life: 24 months
Product Size: 50 mL
High Throughput Compatibility: Manual Protocols
Shipping Condition: Room Temperature
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Contains: 50 mL Dynabeads® Protein G
Storage: 2°C to 8°C |