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Dynabeads® Protein G Immunoprecipitation Kit
The Dynabeads® Protein G Immunoprecipitation Kit provides a faster and easier alternative to Sepharose® or agarose slurry for immunoprecipitation (IP). The kit supplies Dynabeads® Protein G (uniform, 2.8 µm superparamagnetic beads with recombinant Protein G covalently coupled to the surface) and the buffers needed to perform small-scale immunoprecipitation (IP) and co-IP.
• Simple, short ~30 minute procedure
• Significantly reduces background caused by non-specific binding
• No columns, centrifugations, or time-consuming pre-treatment of your samples are needed
• Gentle separation preserves native protein conformations and large-protein complexes
Magnetic bead-based separation is rapid and easy to perform
Antibody is added to the Dynabeads® Protein G. During a short incubation, the antibody binds to the Dynabeads® via their Fc-region. The tube is then placed on a magnet, where the beads
Form: Bead
Ligand: Protein G
Shelf Life: 24 months
Product Size: 40 reactions
Bead Diameter: 2.8 µm
Concentration: 30 mg⁄ml
Sample Type (Specific): Clinical Sample,
Tissue Digests,
Supernatants,
Cell Extracts,
Serum,
Cell Cultures
High Throughput Compatibility: High Throughput-Compatible
Shipping Condition: Room Temperature
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Contains:
• 2 ml Dynabeads® Protein G (30 mg/ml)
• 16 mL Antibody (Ab) Binding and Washing Buffer
• 28 mL Washing Buffer
• 1 mL Elution Buffer
Store at 2-8°C |