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FlowCellect™ Mouse FoxP3 Treg Identification Kit
Description:
FlowCellect™ Mouse FoxP3 Treg Identification Kit
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
Millipore’s FlowCellect™ Mouse FoxP3 Treg Identification Kit is designed to enable a researcher a quick and easy way to detect FoxP3 expression in mouse CD4+ T-cells.
Millipore’s FlowCellect™ Mouse FoxP3 Treg Identification Kit contains sufficient reagents for 25 2-color tests. The kit includes two optimized fluorescent labeled antibodies and buffers necessary for cell preparation and analysis. Detailed assay instructions are included to assist in analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data.
Background Information:
Regulatory T-cells, or Treg cells are an important subset of CD4+ T-cells. The identification of Tregs has shown that they are critical in the control of normal immune responses. Treg cells act to suppress immune responses and keep the immune system in check by inhibiting cell proliferation and cytokine production. Treg cells represent 2-10% of the total peripheral CD4+ T-cells and are characterized by the expression of the forkhead box transcription factor, FoxP3. FoxP3 is a master regulator of Treg development and function. Treg cells develop from a common naïve CD4+ T-cell precursor and expression of FoxP3 is induced in the presence of TGFβ and IL-2. Lack of FoxP3 in humans leads to the fatal autoimmune disease, IPEX. In addition, studies with the FoxP3-deficient mouse model, also known as the scurfy mouse, have shown that FoxP3 expression is profoundly important for the regulation of the immune system and the development of Tregs cells.
Applications:
This FlowCellect Mouse FoxP3 Treg Identification Kit for flow cytometry is designed to enable a researcher a quick & easy way to detect FoxP3 expression in mouse CD4+ T-cells.
Key Applications:
Flow Cytometry
Species Reactivity:
Mouse
Usage Statement:
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Conditions:
4-8°C for antibodies and buffers
Product Name:
FlowCellect™ Mouse FoxP3 Treg Identification Kit
Alternate Names:
Flow Cellect
Flow Cytometry Software Type:
ExpressPro & inCyte
Antibody Type:
Monoclonal Antibody
Flow Cytometry Market:
Research
Quality Assurance:
Lot to lot in-process testing on all kit components
Components:
1. 20X Anti-CD4 clone GK1.5-PerCP : (Part No. CS205790) 1 vial containing 125 µL.
2. 20X Anti-FoxP3 clone 3G3 AlexaFluor®647: (Part No.CS205800) 1 vial containing 125 µL.
3. Fixation Buffer: (Part No. CS202122) One bottle containing 13 mL.
4. 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL.
5. 5X Assay Buffer: (Part No. CS202124) One 55 mL bottle
6. Permeabilization Buffer Concentrate: (Part No. CS205792) one bottle containing 10 mL buffer.
Host:
Mouse
Detection Methods:
Fluorescent |