|
FlowCellect™ Human Lymphocyte ZAP-70 Characterization Kit
Close
PRODUCT FAMILY INFORMATION
Flow Cytometry Kits & Antibodies
Millipore's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
FlowCellect Kits are EMD Millipore's proprietary multiparameter flow cytometry kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments. In addition, EMD Millipore's line of Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody.
» More…
Description:
FlowCellect™ Human Lymphocyte ZAP-70 Characterization Kit
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
We have developed a multi-parameter flow cytometry assay for ZAP-70 protein expression analysis and characterization of human lymphocytes. Millipore’s FlowCellect™ Human Lymphocyte ZAP-70 Characterization Kit includes three directly conjugated antibodies: CD5 FITC, CD19 APC, and ZAP-70 PE, along with optimized fixation, permeabilization, wash, and assay buffers to provide researchers the ability to phenotypically distinguish cell types.
Antibodies against CD5 and CD19 are provided in the kit to phenotype cells by their surface marker expression. CD5 is normally a pan-T-cell marker, while CD19 is a mature B-cell marker. Some B-cell malignancies express both of these molecules on their surface, and therefore can be distinguished from the two aforementioned cell types.
ZAP-70 is a molecule involved in T-cell activation, which can also be expressed within B-cells at levels correlating to disease severity. The combinatorial staining of cells for all three of these markers assists the researcher studying the molecular biology of T-cell activation or B-cell disease states.
Sufficient reagents are provided for 25 3-color samples. The kit includes all optimized fluorescent labeled antibodies and buffers necessary for cell preparation and analysis.
View All »
Background Information:
Characterization of lymphocytes and lymphocyte sub-types is essential to understanding the complex nature of the immune system. Activation to antigens, suppression of normal immune activation, and disease states can affect the phenotype of lymphocytes. Multi-parameter phenotypic analysis allows for a greater understanding of the molecular biology associated with these intricate processes.
ZAP-70 is a cytoplasmic protein tyrosine kinase that plays a critical role in the events involved in initiating T-cell responses by the antigen receptor. Due to its role in T-cell activation, lack of ZAP-70 expression in T-cells can lead to immune deficiencies as a result of decreased response to antigens.
CD5 is expressed on the cell surface of T-cells, NK cells, and has been identified as a marker for some B-Cell malignancies. Normal B-cells express CD19 but do not express CD5 or ZAP-70 which are typically associated with T-cells; however, there are some disease states in which B-cells have been found to express all three markers. In these instances, multi-parameter phenotypic analysis allows for the monitoring of marker expression on lymphocytes within the peripheral blood.
The ability |