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Actin Cytoskeleton / Focal Adhesion Staining Kit
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PRODUCT FAMILY INFORMATION
Cell Based Assays
Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell, and cell-based activity assays. Study Apoptosis, Angiogensis, Adhesion and more.
EMD Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell and cell-based activity assays that mimic native environments for direct and indirect detection of cellular response.
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Description:
Actin Cytoskeleton / Focal Adhesion Staining Kit
Trade Name:
Chemicon (Millipore)
Qty/Pk:
100 assays
Product Overview:
The Actin Cytoskeleton and Focal Adhesion Staining Kit consists of three components (TRITC-conjugated phalloidin, anti-Vinculin and DAPI) for the immunofluorescent staining of actin filaments in the cytoskeleton, focal contacts as well as the nucleus of the cells.
Reagents and materials supplied in the Actin Cytoskeleton and Focal Adhesion Staining Kit are sufficient for 100 tests (including controls).
Introduction
The actin cytoskeleton is a highly dynamic network composed of actin polymers and a large variety of associated proteins. The functions of the actin cytoskeleton is to mediate a variety of essential biological functions in all eukaryotic cells, including intra- and extra-cellular movement and structural support. To perform these functions, the organization of the actin cytoskeleton must be tightly regulated both temporally and spatially. Many proteins associated with the actin cytoskeleton are thus likely targets of signaling pathways controlling actin assembly. Actin cytoskeleton assembly is regulated at multiple levels, including the organization of actin monomers (G-protein) into actin polymers and the superorganization of actin polymers into a filamentous network (F-actin - the major constituent of microfilaments) [1]. This superorganization of actin polymers into a filamentous network is mediated by actin side-binding or cross-linking proteins [2, 3, 4]. Actin cytoskeleton is a dynamic structure that rapidly changes shape and organization in reponse to stimuli and cell cycle progression. Therefore, a disruption of its normal regulation may lead to cell transformations, hence cancer. Transformed cells have been shown to contain less F-actin than untransformed cells and exhibit atypical coordination of F-actin levels to the cell cycle [5]. Orientational distribution of actin filaments within a cell is an important determinant of cellular shape and motility. Therefore,
Focal adhesion and adherens junctions are membrane-associated complexes that serve as nucleation sites for actin filaments and as cross-linkers between the cell exterior, plasma membrane and actin cytoskeleton [6]. The function of focal adhesions are structural, linking the ECM on the outside to the actin cytoskeleton on the inside. They are also sites of signal transduction, initiating of signaling pathways in response to adhesion. Focal adhesions consist of integrin-type receptors that are attached to the extracellular matrix and are intracellularly associated with protein complexes containing vinculin (universal focal adhesion marker), talin, a-actinin, paxillin, tensin, zyxin and focal adhesion kinase (FAK) [7,8].
CHEMICON's Actin Cytoskeleton and Focal Adhesion Staining Kit (Catalog number FAK100) is a very sensitive immunocytochemical tool that contains fluorescent-labeled phalloidin (TRITC-conjugated phalloidin) to map the local orientation of actin filaments within cell and a mouse monoclonal antibody to Vinculin that is very specific for the staining of focal contacts in cells.
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Applications:
The Actin Cytoskeleton & Focal Adhesion Staining Kit consists of TRITC-conjugated phalloidin, anti-Vinculin & |