|
QCM Chemotaxis Cell Migration Assay, 24-well (8 μm), colorimetric
Close
PRODUCT FAMILY INFORMATION
Cell Based Assays
Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell, and cell-based activity assays. Study Apoptosis, Angiogensis, Adhesion and more.
EMD Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell and cell-based activity assays that mimic native environments for direct and indirect detection of cellular response.
» More…
Description:
QCM Chemotaxis Cell Migration Assay, 24-well (8 μm), colorimetric
Trade Name:
QCM
Chemicon (Millipore)
Qty/Pk:
1 plate
Product Overview:
Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here)
Introduction
Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation. Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. Recently, a fluorescence blocking membrane insert was introduced to address these issues; however, this approach requires labeling of the cells with Calcein-AM and extensive washing to remove free Calcein before cell migration. The effect of this treatment on cell behavior/migration remains questionable.
The CHEMICON® QCM™ 24-well Cell Migration Assay (ECM508) eliminates cell pre-labeling and manual counting. The 24-well insert and colorimetric detection format allows for quantitative comparison of multiple samples.
In the CHEMICON® QCM™ 24-well Migration Assay, cells that have migrated to the bottom of the insert membrane are stained. The stain is then extracted and transferred to a 96-well microtiter plate for colorimetric measurement.
The CHEMICON® QCM™ 24-well Migration Assay provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells.
The CHEMICON® QCM™ 24-well Migration Assay utilizes an 8 mm pore size, as this is appropriate for most cell types. This pore size supports optimal migration for most epithelial and fibroblast cells; however, it is not appropriate for lymphocyte migration experiments. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis.
In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:
· QCM™ 8μm 96-well Chemotaxis Cell Migration Assay (ECM510)
· QCM™ 5μm 96-well Chemotaxis Cell Migration Assay (ECM512)
· QCM™ 3μm 96-well Chemotaxis Cell Migration Assay (ECM515)
· QCM™ 96-well Cell Invasion Assay (ECM555)
· QCM™ 96-well Collagen-based Cell Invasion Assay (ECM556)
· 24-well Insert Cell Migration and Invasion Assay Systems
· CytoMatrix™ Cell Adhesion strips (ECM protein coated)
· QuantiMatrix™ ECM protein ELISA kits
Test Principle
The CHEMICON® QCM™ 24-well Cell Migration Assay is performed in a Migration Chamber, based on the Boyden chamber principle. Each kit contains 24 inserts; each insert utilizes an 8 mm pore size polycarbonate membrane, as this is appropriate for most cell types. This pore size supports optimal migration for most epithelial and fibroblast cells; however, it is not appropriate for lymphocyte migration expe |