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QCM Chemotaxis Cell Migration Assay, 24-well (5 μm), fluorimetric
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Cell Based Assays
Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell, and cell-based activity assays. Study Apoptosis, Angiogensis, Adhesion and more.
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Description:
QCM Chemotaxis Cell Migration Assay, 24-well (5 μm), fluorimetric
Trade Name:
QCM
Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here)
Introduction
The CHEMICON® QCM™ Chemotaxis 5 μm 24-well Migration Assay is performed in a Migration Chamber, based on the Boyden chamber principle. The 5 μm pore size of this assay's Boyden chambers is appropriate for studying monocyte/macrophage migration. The quantitative nature of this assay is useful for screening of pharmacological agents. Each kit provides sufficient materials for the evaluation of 24 samples.
Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammationIn immune responses, monocyte migration is critical for providing immunological defense and eliminating infectious agents. Monocytes (macrophage precursors) circulate the blood until encountering specific chemotactic factors that initiate the migration process. Once engaged, monocytic cells transverse the vascular endothelium in a multi-step mechanism by: A) E-selectin mediated monocyte rolling B) cell adhesion C) transmigration into the subendothelium. During this migration and entering the tissue, monocytes develop/differentiate into mature macrophages, increasing in size, phagocytic activity, and proteolytic enzyme secretion. Once at the site of infection, mature macrophage respond to infection by phagocytosis, cytokine secretion, respiratory burst, T-cell antigen presentation, and digestive enzyme secretion.
Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. However, current methods of analysis are time-consuming and tedious, involving cotton swabbing of non-migrated cells on the topside of insert, manual staining and counting. Recently, a fluorescence blocking membrane insert was introduced to address these issues; however, this approach requires labeling of the cells with Calcein-AM and extensive washing to remove free Calcein before cell migration. The effect of this treatment on cell behavior/migration remains questionable.
The Chemicon QCM™ Chemotaxis 5 μm 24-well Migration Assay does not require cell labeling, scraping, washing, or counting. The 24-well inserts and homogenous fluorescence detection format allow for convenient screening and quantitative comparison of multiple samples.
In the Chemicon QCM™ Chemotaxis 5 μm 24-well Migration Assay, migratory cells on the bottom of the insert membrane are dissociated from the membrane when incubated with the Cell Detachment Solution. These cells are subsequently lysed and detected by the patented CyQUANT® GR dye (Molecular Probes). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids.
Most migration assays utilize an 8 μm pore size, as this is appropriate for most cell types, e.g. epithelial and fibroblast cells. The Chemicon QCM™ Chemotaxis 5 μm 24-well Migration Assay utilizes a 5 μm pore size, which is appropriate for monocyte/macrophage |