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Phospho-GSK-3β (Ser9) STAR ELISA Kit
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PRODUCT FAMILY INFORMATION
GSK-3 Antibodies, Proteins, Inhibitors
Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways.
Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways. EMD Millipore’s GSK-3 antibodies, proteins and inhibitors are based on the expertise of Upstate, Chemicon, and Calbiochem. Our GSK-3 reagents have been well validated and published. See the ordering tab for a comprehensive list of our GSK-3 products and direct links to their technical pages for data, references and related products.
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STAR ELISA Kits
Upstate STAR (Signal Transduction Assay Reaction) ELISA kits are a fast, sensitive method to detect the relative amounts of total and activated targets through the use of phospho-specific antibodies.
Upstate STAR (Signal Transduction Assay Reaction) ELISA kits are a fast, sensitive method to detect the relative amounts of total and activated targets through the use of phospho-specific antibodies.
» More…
Description:
Phospho-GSK-3β (Ser9) STAR ELISA Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
96 assays
Background Information:
I. TEST PRINCIPLE The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The GSK-3β plate is coated with a specific mouse monoclonal GSK-3&beta capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incubated in the microwells allowing GSK-3β antigen to be captured in the plate wells. The plate is then washed to remove any non-bound unspecific material. The wells are then incubated with a specific rabbit anti-GSK-3β antibody to detect the captured phosphor-GSK-3β (Ser9) on the plate well. The unbound detection antibody is washed away followed by incubation with an HRP-conjugated anti-rabbit antibody. This allows for a sensitive enzymatic detection of the sample. After the addition of TMB substrate and stop solution the absorbance is measured at 450 nm using a plate reader. The entire assay takes less than 5 hours to complete with minimal hands-on time. Many of the reagents are supplied in ready-to use formulations for ease of use. The kit also includes a standard that is run as both a positive control and to develop a standard line of detection.
II. GSK-3β BACKGROUND
Glycogen Synthase Kinase-3 differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. When it becomes phosphorylated, the protein becomes inactive. There are two isoforms that exist, GSK-3α and GSK-3β. The function of GSK-3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway that results in the activation of Akt. Akt phosphorylates GSK-3, thereby inactivating it. Glycogen Synthase, GSK-3's downstream substrate, is then rapidly dephosphorylated and activated. Other GSK-3 substrates include Jun (on inhibitory sites), and eIF2B. Detection of GSK-3's phosphorylation status of the Akt site (Ser21) on GSK-3 is suitable for surrogate assays of the activation state of the pathway.
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Applications:
This Phospho-GSK-3β (Ser9) STAR ELISA Kit is used to measure & quantify GSK-3β levels.
Key Applications:
ELISA
Species Reactivity:
Human
Mouse
Rat
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying |