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GSK-3β STAR ELISA Kit
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PRODUCT FAMILY INFORMATION
GSK-3 Antibodies, Proteins, Inhibitors
Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways.
Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways. EMD Millipore’s GSK-3 antibodies, proteins and inhibitors are based on the expertise of Upstate, Chemicon, and Calbiochem. Our GSK-3 reagents have been well validated and published. See the ordering tab for a comprehensive list of our GSK-3 products and direct links to their technical pages for data, references and related products.
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STAR ELISA Kits
Upstate STAR (Signal Transduction Assay Reaction) ELISA kits are a fast, sensitive method to detect the relative amounts of total and activated targets through the use of phospho-specific antibodies.
Upstate STAR (Signal Transduction Assay Reaction) ELISA kits are a fast, sensitive method to detect the relative amounts of total and activated targets through the use of phospho-specific antibodies.
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Description:
GSK-3β STAR ELISA Kit
Trade Name:
STAR
Upstate (Millipore)
Qty/Pk:
96 assays
Background Information:
The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The GSK-3β plate is coated with a specific mouse monoclonal anti-GSK-3β capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incubated in the microwells allowing GSK-3β antigen to be captured in the plate wells. The plate is then washed to remove any non-bound unspecific material. The wells are then incubated with a specific rabbit anti-GSK-3β antibody to detect the captured GSK-3β on the plate well. The unbound detection antibody is washed away followed by incubation with an HRP-conjugated anti-rabbit antibody. This allows for a sensitive enzymatic detection of the sample. After the addition of TMB substrate and stop solution the absorbance is measured at 450 nm using a plate reader. The entire assay takes less than 5 hours to complete with minimal hands-on time. Many of the reagents are supplied in ready-to use formulations for ease of use. The kit also includes a standard that is run as both a positive control and to develop a standard line of detection.
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Applications:
The GSK-3β colorimetric STAR ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to quantify specific levels of signaling targets in denatured cell extracts.
Key Applications:
ELISA
Species Reactivity:
Human
Mouse
Rat
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMe |