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HAT Assay Reagent Kit
Description:
HAT Assay Reagent Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
Accessory reagent kit for the analysis of histone acetyltransferase activity.
Applications:
Accessory reagent kit for the analysis of histone acetyltransferase activity.
Key Applications:
Histone Acetyltransferase Assay (HAT)
Application Notes:
Nuclear Extract (source of HAT enzyme activity): Prepare nuclear extracts using a modified protocol of Dignam, et al., Nucleic Acids Res. 22: 1475, 1983 (recommended, but other methods may be used). Use 1- 20μl per assay point in the HAT Reaction Cocktail below. Optimal nuclear extract concentration will need to be determined empirically.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. This gene encodes a histone acetylase that contains A, B, and D motifs, present in many N-acetyltransferases, including those that acetylate substrates other than histones. Alternatively spliced transcript variants that encode different isoforms have been identified for this gene.
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UniProt Summary:
FUNCTION: SwissProt: O14929 # May play a role in telomeric silencing. Acetylates soluble but not nucleosomal H4 at 'Lys-5' and 'Lys-12' and acetylates histone H2A at 'Lys-5'. HAT1 has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG.
SIZE: 419 amino acids; 49513 Da
SUBUNIT: Heteromer of HAT1 and p46/HAT2 subunits.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear in S-phase cells and cytoplasmic.
SIMILARITY: SwissProt: O14929 ## Belongs to the HAT1 family.
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Brand Family:
Upstate
Gene Symbol:
HAT1
Product Name:
HAT Assay Reagent Kit
Quality Assurance:
Routinely evaluated by incubating pCAF, active (14-309) with Core Histones (13-107B). Reactions were terminated at indicated times and acetyl incorporation was measured.
Components:
HAT Assay Buffer, 5X
Core Histones
P81 Phosphocellulose squares
Acetyl-CoA
UniProt Number:
O14929
Packaging:
Kit capacity: 100 assays
Entrez Gene Number:
NM_001033085.1
NM_003642.2 |