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PKA Assay Kit
Description:
PKA Assay Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Applications:
The radioactive PKA Assay Kit is designed to measure the phosphotransferase activity of PKA (cAMP-dependent Protein Kinase) in immunoprecipitates & column fractions.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
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UniProt Summary:
FUNCTION: SwissProt: P17612 # Phosphorylates a large number of substrates in the cytoplasm and the nucleus.
SIZE: 351 amino acids; 40590 Da
SUBUNIT: A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm (By similarity).
TISSUE SPECIFICITY: Isoform 2 is sperm specific.
PTM: Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively (By similarity).
SIMILARITY: SwissProt: P17612 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain.
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Brand Family:
Upstate
Gene Symbol:
PRKACA
MGC102831
MGC48865
PKACA
Product Name:
PKA Assay Kit
Analytes Available:
PKA
Quality Assurance:
Routinely evaluated by phosphorylation of Kemptide.
Components:
Kemptide, 1mM (Cat.# 20-199)
cAMP (Cat.# 20-198)
PKC/CaMK Inhibitor Cocktail (Cat.# 20-119)
PKA Inhibitor Peptide (Cat.# 20-120)
Assay Dilution Buffer, 5X (Cat.# 20-145)
Magnesium/ATP Cocktail (Cat.# 20-113)
P81 Phosphocellulose Squares (Cat.# 20-134)
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UniProt Number:
P17612
Detection Methods:
Radioactive
Radioactive
Entrez Gene Number:
NM_002730.3
NM_207518.1 |