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Casein Kinase 2 Assay Kit
Description:
Casein Kinase 2 Assay Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Applications:
Measures the phosphotransferase activity of Casein Kinase 2 in immunoprecipitates & column fractions based on phosphorylation of a specific substrate using the transfer of the gamma-phosphate of [g-32P] ATP by CK-2 kinase.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus.
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UniProt Summary:
FUNCTION: SwissProt: P67870 # Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit.
SIZE: 215 amino acids; 24942 Da
SUBUNIT: Tetramer composed of an alpha subunit, an alpha' subunit and two beta subunits. Interacts with CD163. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation.
PTM: Phosphorylated by alpha subunit.
SIMILARITY: SwissProt: P67870 ## Belongs to the casein kinase 2 subunit beta family.
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Brand Family:
Upstate
Gene Symbol:
CSNK2B
Phosvitin
CK2B
CSK2B
MGC138224
MGC138222
CK2N
G5a
phosvitin
G5A
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Product Name:
Casein Kinase 2 Assay Kit
Analytes Available:
Casein Kinase 2
Quality Assurance:
For use to measure the phosphotransferase activity of Casein Kinase 2 (CK-2) in immunoprecipitates and column fractions based on phosphorylation of a specific substrate (CK-2 substrate peptide) using the transfer of the gamma-phosphate of [g-32P] ATP by CK-2 kinase
Components:
Casein Kinase 2 Substrate Peptide (Cat.# 12-330)
Assay Dilution Buffer I (ADBI) (Cat.# 20-108)
PKA Inhibitor Cocktail (Cat.# 20-114)
Magnesium/ATP Cocktail (Cat.# 20-113)
P81 Phosphocellulose Squares (Cat.# 20-134)
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UniProt Number:
P67870
Detection Methods:
Radioactive
Radioactive
Packaging:
Kit capacity: 200 assays
Entrez Gene Number:
NM_001320.5 |