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ChIPAb+ Acetyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set
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PRODUCT FAMILY INFORMATION
Histone H3 Antibodies, Proteins and Kits
Millipore’s Histone H3 antibodies demonstrate specificity against histone H3. See below for acetyl-, methyl-, phospho- histone H3 Antibodies and Proteins, based on the expertise of Upstate & Chemicon.
Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Species Reactivity Key Applications Host Format Antibody Type
H WB, ChIP Rabbit Affinity Purified Polyclonal Antibody
Description:
ChIPAb+ Acetyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set
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Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Acetyl-Histone H3 (Lys4) set includes the Acetyl-Histone H3 (Lys4) antibody, a negative control antibody (normal rabbit IgG), and qPCR primers which amplify a 166 bp region of human GAPDH. The Acetyl-Histone H3 (Lys4) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Acetyl-Histone H3 (Lys4)-associated chromatin.
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Specificity:
Recognizes Histone H3 acetylated on Lys4.
Molecular Weight:
~17 kDa
Epitope:
Acetylated Lys4
Immunogen:
KLH-conjugated, synthetic peptide containing the sequence …RTAcKQ… in which AcK corresponds to acetyl lysine 4 of human histone H3.
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.
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Species Reactivity:
Human
Species Reactivity Note:
Human. Wide range of cross-reactivity expected based on sequence homology.
Application Notes:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µL of Normal rabbit IgG or 4 µL Anti-Acetyl-Histone H3 (Lys4) and the Magna ChIP™ A Kit (Cat. # 17-610).
Successful immunoprecipitation of Acetyl-Histone H3 (Lys4) associated DNA fragments was verified by qPCR using Control Primers as a positive locus on the GAPDH promoter, and β-Globin primers as a negative locus. (Please see figures).
Data is presented as percent input of each IP sample relative to i |