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ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set
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PRODUCT FAMILY INFORMATION
Histone H2A Antibodies, Proteins and Kits
Millipore’s Histone H2A antibodies demonstrate specificity against linker histone H2A and its modifications. Millipore’s Histone H2A specific antibodies, proteins, and kits are presented below.
EMD Millipore’s Histone H2A antibody demonstrates specificity against linker histone H2A. See below for data and references for EMD Millipore’s Histone H2A antibodies, proteins and kits. All EMD Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Species Reactivity Key Applications Host Format Antibody Type
H ChIP, WB Sheep Affinity Purified Polyclonal Antibody
Description:
ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set
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Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Histone H2A.Z set includes the Histone H2A.Z antibody, a negative control antibody (normal sheep IgG), and qPCR primers which amplify a 166 bp region of human GAPDH. The Histone H2A.Z and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Histone H2A.Z -associated chromatin.
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Specificity:
Recognizes Histone H2A.Z, Mr ~15 kDa.
Molecular Weight:
~15 kDa
Epitope:
C-terminus
Immunogen:
Linear peptide corresponding the C-terminal amino acids 113-127 of human Histone H2A.Z.
Background Information:
H2A.Z, also known as H2AFZ, is a variant of histone H2A variant that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.
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Species Reactivity:
Human
Species Reactivity Note:
Human. Wide species reactivity is expected.
Predicted to cross-react with zebrafish, mouse, chicken, rat, rabbit, and Xenopus based on sequence homology.
Application Notes:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µg of either Normal Sheep IgG or 1 µg of Anti-Histone H2A.Z and the Magna ChIP™ G Kit (Cat. # 17-611).
Successful immunoprecipitation of Histone H2A.Z associated DNA fragments was verified |