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Anti-Stat3 Antibody
Description:
Anti-Stat3 Antibody
Replaces:
AB3162
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Specificity:
This antibody recognizes STAT3 near the C-terminus.
Molecular Weight:
~ 88 kDa observed.
Epitope:
Near C-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to human STAT3 near the C-terminus.
Background Information:
This protein is a member of the family of STAT proteins (Signal Transduction and Activators of Transcription). These proteins are latent cytoplasmic transcription factors with the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. The Stat3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN-a, IL-10, IL-6, IL-11, onM, LIF, CNTF, G-CSF, IL-12, IL-2, GH, EGF, PDGF, and CSF-1. Stat3 is not activated by IFN beta. One active Stat3 translocates to the nucleus and binds to DNA as either a homodimer or as a heterodimer with Stat1. Transcription factors associated with Stat3 include cJun and cyclic AMP responsive enhancer binding protein (CREB). Stat3 interacts with the APRE (acute phase response element) and the SIE (c-sis inducible element). These proteins are latent cytoplasmic transcription factors with the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue.
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Species Reactivity:
Human
Mouse
Rat
Bovine
Pig
Primate
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Species Reactivity Note:
Demonstrated to react with human, mouse, and rat. Predicted to react with bovine, porcine, and primate based on 100% sequence homology.
Application Notes:
Western Blot (SNAP ID) Analysis: 5 µg/mL from a representative lot detected STAT3 on 10 µg of HeLa cell lysate.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated STAT3 from 500 µg of HeLa RIPA lysate.
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected STAT3 on 10 µg of HeLa cell lysate.
Purification Method:
Protein A
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P40763
Entrez Gene Number:
NP_644805
Gene Symbol:
APRF
HIES
STAT3
Alternate Names:
Acute-phase response factor
DNA-binding protein APRF
signal transducer and activator of transcription 3
signal transducer and activator of transcription 3
(acute-phase response factor)
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
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