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Anti-Frizzled-4 Antibody
Description:
Anti-Frizzled-4 Antibody
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Specificity:
This antibody recognizes Frizzled-4 at the extracellular domain.
Molecular Weight:
~ 62 kDa observed
Epitope:
Extracellular domain
Immunogen:
GST-tagged recombinant protein corresponding to the extracellular domain of rat Frizzled-4.
Background Information:
Frizzled-4 is a seven pass membrane protein that is a member of the G-protein coupled receptor Fz/SUMO family. It is a Wnt protein receptor and is a critical component in the vascularization of the retina, where it can be activated either by Wnt protein-binding or by norrin (NDP). Both activation pathways lead to the upregulated accumulation of beta-catenin and the inducement of transcriptional pathways mediated by LEF/TCF. Frizzled 4 reportedly interacts with NDP and MAGI3, and is part of complex that includes, NDP, FZD4, and TSPAN12.
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Species Reactivity:
Human
Mouse
Rat
Canine
Chimpanzee
Primate
Rhesus Macaque
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Species Reactivity Note:
Demonstrated to react with human, mouse, and rat. Predicted to react with canine, chimpanzee, primate, and rhesus macaque based on 100% sequence homology.
Application Notes:
Western Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected Frizzled-4 on 10 µg of rat heart tissue lysate.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Frizzled-4 in ductal carcinoma in situ tissue.
Control:
Human heart tissue lysate
Quality Assurance:
Evaluated by Western Blot in human heart tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Frizzled-4 on 10 µg of human heart tissue lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9QZH0
Entrez Gene Number:
NP_072145
Gene Symbol:
FZD4S
CD344
FzE4
FEVR
hFz4
Fz-4
EVR1
GPCR
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Alternate Names:
frizzled homolog 4
frizzled-4
WNT receptor frizzled-4
exudative vitreoretinopathy 11
CD344 antigen3
frizzled homolog 4
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
Putative Wnt receptor; may play a role in the Wnt signaling pathway [RGD].
UniProt Summary:
FUNCTION: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LE |