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Anti-Giα2 Antibody, clone L5
Recommended Replacement for: 05-14013 is a recommended replacement for MAB3077
Species Reactivity Key Applications Host Format Antibody Type
R, B, M, H, Gp, R WB, IH(P) Mouse Purified Monoclonal Antibody
Description:
Anti-Giα2 Antibody, clone L5
Replaces:
05-14013 is a recommended replacement for MAB3077
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Specificity:
This antibody recognizes Giα-2.
Molecular Weight:
Labels a single protein band of 39-42 kDa in bovine and rat membranes.
Immunogen:
Recombinant Giα-2, purified by the method of Linder (J. Biol. Chem. [1990] 265, 8243).
Clone:
L5
Isotype:
IgG2b
Species Reactivity:
Rat
Bovine
Mouse
Human
Guinea Pig
Rat
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Species Reactivity Note:
Strong reactivity with bovine (100%), mouse (69%), rat (69%), human (62%) and guinea pig (43%). Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB.
Application Notes:
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer
Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes
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Control:
Huvec cell lysate
Quality Assurance:
Routinely evaluated by Western Blot on Huvec lysates.
Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates.
Purification Method:
Purified
Presentation:
Purified mouse monoclonal IgG2b, in 0.02 M phosphate buffer, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide.
Storage Conditions:
Maintain at 2-8°C for 1 year from date of receipt.
UniProt Number:
P04899
Entrez Gene Number:
NM_002070.2
Gene Symbol:
GNAI2
GNAI2B
WUGSC:H_LUCA15.1
H_LUCA15.1
H_LUCA16.1
GIP
WUGSC:H_LUCA16.1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P04899 # Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli.
SIZE: 355 amino acids; 40451 Da
SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GPSM1 (By similarity). Interacts with UNC5B.
SIMILARITY: SwissProt: P04899 ## Belongs to the G-alpha family. G(i/o/t/z) subfamily.
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Product Name:
Anti-Giα2 Antibody, clone L5
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
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