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Anti-FGF-2/basic FGF Antibody, clone bFM-2
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REFERENCES
Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-β3 in crush-injured skeletal muscle.
Mai-Khoi Q DO,Takahiro Suzuki,Borjigin Gerelt,Yusuke Sato,Wataru Mizunoya,Mako Nakamura,Yoshihide Ikeuchi,Judy E Anderson,Ryuichi Tatsumi (2012) Animal science journal = Nihon chikusan Gakkaihō.83
Increased fibroblast telomerase expression precedes myofibroblast α-smooth muscle actin expression in idiopathic pulmonary fibrosis.
Daniel Reis Waisberg,Edwin Roger Parra,Jo Barbas-Filho,Sandra Fernezlian,Vera Luiza Capelozzi (2012) Clinics (S.67
Differential Gene Expression in Eyecup and Retina of a Mouse Model of Stargardt-like Macular Dystrophy (STGD3).
Kuny, Sharee, et al. (2012) Invest. Ophthalmol. Vis. Sci., 53: 664-75 (2012)
Preferential accumulation and export of high molecular weight FGF-2 by rat cardiac non-myocytes.
Jon-Jon Santiago,Xin Ma,Leslie J McNaughton,Barbara E Nickel,Brian P Bestvater,Liping Yu,Robert R Fandrich,Thomas Netticadan,Elissavet Kardami (2011) Cardiovascular research.89
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Species Reactivity Key Applications Host Format Antibody Type
H, B, M, R IHC, RIA, WB Mouse Purified Monoclonal Antibody
Description:
Anti-FGF-2/basic FGF Antibody, clone bFM-2
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Trade Name:
Upstate (Millipore)
Product Overview:
This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity. Upstate recommends anti-FGF-2/basic FGF, neutralizing (Catalog #05-117) for neutralizing activity.
Specificity:
This antibody detects FGF-2/basic FGF.
Molecular Weight:
17.5 kDa
Immunogen:
Purified bovine brain basic FGF (FGF-2, HBGF-2). Clone bFM-2.
Clone:
bFM-2
Isotype:
IgG1κ
Background Information:
FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
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Species Reactivity:
Human
Bovine
Mouse
Rat
Species Reactivity Note:
This antibody is highly specific for basic FGF from bovine, human, rat, and mouse sources and does not cross-react with bovine acidic FGF (FGF-1, HBGF-1).
Important Note: This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity (Uteza, Y., 1999). Upstate recommends anti-FGF-2/basic FGF, clone bFM-1 (neutralizing) (Catalog # 05-117) for neutralizing activity.
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Application Notes:
Immunohistochemistry:
Previous lots of this antibody have been shown to immunostain frozen brain sections fixed with ice-cold, ethanol:acetic acid [95:5]. This antibody has also been used at 10 μg/mL to stain paraffin-embedded sections (Matsuzaki, K., 1989).
Control:
Fetal liver or kidney tissue.
Quality Assurance:
Evaluated on basic FGF (Catalog #01-114).
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected 100 ng FGF-2/basic FGF, carrier-free (Catalog # 01-114).
Purification Meth |