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Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1
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REFERENCES
Membrane vesicles containing matrix metalloproteinase-9 and fibroblast growth factor-2 are released into the extracellular space from mouse mesoangioblast stem cells.
Candela, Maria Elena, et al. (2010) J. Cell. Physiol., 224: 144-51 (2010)
A novel mechanism of sequestering fibroblast growth factor 2 by glypican in lipid rafts, allowing skeletal muscle differentiation.
Jaime Gutiérrez,Enrique Brandan (2010) Molecular and cellular biology.30
Postischemic intraventricular administration of FGF-2 expressing adenoviral vectors improves neurologic outcome and reduces infarct volume after transient focal cerebral ischemia in rats.
Takuji Watanabe, Yasuaki Okuda, Naosuke Nonoguchi, Ming Zhu Zhao, Yoshinaga Kajimoto, Daisuke Furutama, Hiroyuki Yukawa, Masa-Aki Shibata, Yoshinori Otsuki, Toshihiko Kuroiwa, Shin-Ichi Miyatake (2004) Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.24:1205-13
Monoclonal antibodies against heparin-binding growth factor II/basic fibroblast growth factor that block its biological activity: invalidity of the antibodies for tumor angiogenesis.
Matsuzaki, K, et al. (1989) Proc. Natl. Acad. Sci. U.S.A., 86: 9911-5 (1989)
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Species Reactivity Key Applications Host Format Antibody Type
B, H, M, R NEUT, RIA, not WB Mouse Purified Monoclonal Antibody
Description:
Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes the non-denatured form of FGF-2/basic FGF; does not cross-react with bovine acidic FGF.
Molecular Weight:
17.5 kDa
Epitope:
a.a. 5-24
Immunogen:
Purified bovine brain basic FGF (HBGF-2). Clone bFM-1.
Clone:
bFM-1
Isotype:
IgG1κ
Background Information:
Basic fibroblast growth factor, also known as bFGF or FGF2, is a member of the fibroblast growth factor family.
In normal tissue, basic fibroblast growth factor is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide.
It has been hypothesized that, during both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF, thus mediating the formation of new blood vessels, a process known as angiogenesis.
Additionally, bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined.
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Species Reactivity:
Bovine
Human
Mouse
Rat
Species Reactivity Note:
This antibody is highly specific for the non-denatured form of FGF-2/basic FGF from bovine, human, rat and mouse sources. Does not cross-react with bovine acidic FGF.
Applications Not Recommended:
Western Blotting
Control:
Fetal liver or kidney tissue.
Quality Assurance:
Routinely evaluated by neutralization of greater than 50% of growth of FBHE cells simulated with FGF-2/bFGF.
Neutralization of bFGF Activity Assay:
2.5 µg/mL of this lot of antibody neutralized greater than 40% of growth of FBHE cells simulated with 5 ng/mL of FGF-2/bFGF.
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Purification Method:
Ammonium sulfate precipitation and DEAE-cellulose chromatography
Presentation:
Purified mouse monoclonal IgG1κ in TBS. Protei |