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Anti-Erk1/2 (p44/p42) Antibody, clone MK12
Recommended Replacement for: 05-1152 is a recommended replacement for MAB3054
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PRODUCT FAMILY INFORMATION
Erk (MAP) Kinase Antibodies
Millipore’s Erk Antibodies demonstrate specificity against Erk kinases, p44 and p42 MAP kinases. See below for related products, including proteins & kits based on the expertise of Upstate & Chemicon.
Millipore’s Anti- Erk Antibodies demonstrates specificity against Erk. See below for data, references and related products for Erk. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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MAPK Antibodies, Proteins, Inhibitors, & Assays
Offering hundreds of premium quality antibodies, enzymes, activity assays, and small molecule inhibitors for MAPK signaling research
EMD Millipore offers hundreds of premium quality antibodies, enzymes, activity assays, and small molecule inhibitors for MAPK signaling research. All EMD Millipore products are based on the expertise of Upstate, Chemicon, and Calbiochem.
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Ca, Xn, B, Ch WB, IP, IH(P), ICC Mouse Purified Monoclonal Antibody
Description:
Anti-Erk1/2 (p44/p42) Antibody, clone MK12
Replaces:
05-1152 is a recommended replacement for MAB3054
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Specificity:
ERK1 is the 44 kDa member of the ERK family and shares 85% homology with ERK2. By Western blot, this monoclonal recognizes 44 kDa and 42 kDa proteins from multiple cell lysates including NIH/3T3, RAW264, C2C12, and PC12 as well as tissue samples such as rat pituitary.
Molecular Weight:
This monoclonal recognizes 42 and 44 kDa proteins from rat pituitary.
Immunogen:
Synthetic peptide corresponding to positions 325-345 of rat ERK1.
Clone:
MK12
Isotype:
IgG1
Background Information:
Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively).
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Species Reactivity:
Human
Mouse
Rat
Canine
Xenopus
Bovine
Chicken
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Species Reactivity Note:
Human, Mouse, Rat, Canine, Xenopus, Bovine, and Chicken.
Application Notes:
Immunocytochemistry: A previous lot of this antibody was used in IC.
Immunoprecipitation: A previous lot of this antibody was used in IP.
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of ERK1/2 with Citrate pH 6.0 Epitope Retrieval: Human Placenta
.Erk 1/2 representative staining pattern/morphology in human placenta. Tissue pretreate |