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Anti-VEGF, clone CH-10
Recommended Replacement for: MAB1665
Species Reactivity Key Applications Host Format Antibody Type
H, R WB Mouse Affinity Purified Monoclonal Antibody
Description:
Anti-VEGF Antibody, clone CH-10
Replaces:
MAB1665
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Specificity:
This antibody recognizes human and rat VEGF, other variants not tested.
Molecular Weight:
12/16/19 kDa
Immunogen:
Recombinant mouse VEGF.
Clone:
CH-10
Isotype:
IgG1
Background Information:
VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines.
Species Reactivity:
Human
Rat
Species Reactivity Note:
Reacts with human and rat.
Control:
Jurkat cell lysate
Quality Assurance:
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 1:1000 dilution of this antibody detected VEGF on 10 µg of Jurkat cell lysate.
Purification Method:
Affinity Purfied
Presentation:
Liquid in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6 containing 0.1% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P15692
Entrez Gene Number:
NP_033531.3
Gene Symbol:
MGC70609
MVCD1
VEGF
VEGF-A
VPF
Alternate Names:
Vascular permeability factor
vascular endothelial growth factor
vascular endothelial growth factor A
vascular endothelial growth factor isoform VEGF165
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Entrez Gene Summary:
This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms.
UniProt Summary:
FUNCTION: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to VEGFR2/Kdr but doesn't activate downstream signaling pathways, doesn't activate angiogenesis and inhibits tumor growth.
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