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Anti-Harmartin (TSC1)
Recommended Replacement for: MAB5532
Species Reactivity Key Applications Host Format Antibody Type
H, R WB, IH(P) Mouse Ascites Monoclonal Antibody
Description:
Anti-Harmartin (TSC1) Antibody
Replaces:
MAB5532
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Specificity:
This antibody recognizes Harmartin
Molecular Weight:
Approx. 160 kDa
Immunogen:
Recombinant protein from human Harmartin
Isotype:
IgG1
Background Information:
Harmartin is the gene product of tuberous sclerosis-1 (TSC1). It is thought to have a tumor suppression function and also augments the expression of the TSC2 product tuberin by inhibiting its ubiquitination.
Species Reactivity:
Human
Rat
Species Reactivity Note:
Human, rat
Application Notes:
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Harmartin in human skin tissue, melanocytes.
Control:
NIH/3T3 cell lysate
Quality Assurance:
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 1:500 dilution of this antibody detected Harmartin in 10 µg of NIH/3T3 cell lysate.
Purification Method:
Unpurified ascites fluid
Presentation:
Ascites fluid. Liquid. Contains no preservative.
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
UniProt Number:
Q92574
Entrez Gene Number:
Q92574
Gene Symbol:
KIAA0243
LAM
MGC86987
OTTHUMP00000022439
TSC
hamartin
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Alternate Names:
Tuberous sclerosis 1 protein
tuberous sclerosis 1
tumor suppressor
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling.
SUBUNIT STRUCTURE: Interacts with TSC2, leading to stabilize TSC2. In the absence of TSC2, TSC1 self-aggregates. Interacts with DOCK7. SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note: At steady state found in association with membranes.
TISSUE SPECIFICITY: Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells.
DOMAIN: The C-terminal putative coiled-coil domain is necessary for interaction with TSC2.
PTM: Phosphorylation at Ser-505 does not affect interaction with TSC2. Phosphorylated upon DNA damage, probably by ATM or ATR.
INVOLVEMENT IN DISEASE: Defects in TSC1 are the cause of tuberous sclerosis complex (TSC) [MIM:191100]. The molecular basis of TSC is a functional impairment of the hamartin-tuberin complex. TSC is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. TSC is characterized by hamartomas (benign overgrowths predominantly o |