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Anti-Sulfotyrosine
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REFERENCES
Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum.
Friedman, James, et al. (2012) PLoS ONE, 7: e37648 (2012)
Tyrosine sulfation of the amino terminus of PSGL-1 is critical for enterovirus 71 infection.
Nishimura, Yorihiro, et al. (2010) PLoS Pathog., 6: e1001174 (2010)
Structure and function of broadly reactive antibody PG16 reveal an H3 subdomain that mediates potent neutralization of HIV-1.
Pejchal, Robert, et al. (2010) Proc. Natl. Acad. Sci. U.S.A., 107: 11483-8 (2010)
Species Reactivity Key Applications Host Format Antibody Type
A WB, IP, ELISA Mouse Purified Monoclonal Antibody
Description:
Anti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes sulfated tyrosine residues on various proteins. Does not recognize phosphotyrosine or unmodified tyrosines.
Molecular Weight:
n/a
Epitope:
Sulfated Tyrosine
Immunogen:
This antibody was developed using a phage display library as described in Kehoe et al., 2006. The selection antigens were KAKISDP-DY(SO3)MTGYMDAC and KDKKYATEY(SO3)-EYLDYDFC
Clone:
Sulfo-1C-A2
Isotype:
IgG2aκ
Background Information:
Sulfotyrosine is a common post-translational modification of secretory, plasma membrane, and lysosomal proteins. The sulfation reaction is catalyzed by specific sulfotransferase enzymes in the trans-Golgi-network. Tyrosine sulfation occurs in all multicellular organisms and up to 1% of the tyrosines in the total protein content of a cell can be sulfated. Sulfotyrosines mediates many receptor-ligand binding interactions, including the interaction of P-selectin glycoprotein ligand-1 with P-selectin, factor VIII binding to von Willebrand factor, and interactions between thrombin and hirudin. Sulfotyrosine is also involved in the entry of several parasites and viruses; for example, tyrosine sulfation of the HIV-1 co-receptor CCR5 is required for viral entry into host cells, and tyrosine sulfation of the Duffy antigen/receptor for chemokines is crucial for erythrocyte invasion by the malaria parasite Plasmodium vivax.
This clone was derived from the antibody described in Kehoe et al., 2006.
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Species Reactivity:
All
Species Reactivity Note:
Broad species reactivity. Tested using human, mouse, bovine,and porcine, but the antibody is predicted to recognize any sulfotyrosine, regardless of surrounding amino acid sequence.
Application Notes:
WB, IP, ELISA
Quality Assurance:
Assayed using sulfatase treated bovine fibrinogen vs. untreated fibrinogen.
Purification Method:
Culture supernatant, purified by protein G affinity chromatography
Presentation:
100 μl in 100 mM Tris, 150 mM NaCl, 180 mM Glycine, pH 7.5 containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year from date of receipt when stored at +2-8°C.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
ELISA
Brand Family:
Upstate
Product Name:
Anti-Sulfotyrosine
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μL
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