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Anti-acetyl CoA Carboxylase, clone 7D2.2
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REFERENCES
Human acetyl-CoA carboxylase 1 gene: presence of three promoters and heterogeneity at the 5'-untranslated mRNA region.
Mao, Jianqiang, et al. (2003) Proc. Natl. Acad. Sci. U.S.A., 100: 7515-20 (2003)
Species Reactivity Key Applications Host Format Antibody Type
H, R WB Mouse Purified Monoclonal Antibody
Description:
Anti-acetyl CoA Carboxylase Antibody, clone 7D2.2
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Molecular Weight:
~ 266 kDa Observed
Immunogen:
Histidine-tagged recombinant protein corresponding to human acetyl CoA Carboxylase.
Clone:
7D2.2
Isotype:
IgG2aκ
Background Information:
Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by reversible phosphorylation. The activity of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormone glucagon or epinephrine binds to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl-CoA carboxylase is thus enhanced. Acetyl-CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.
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Species Reactivity:
Human
Rat
Species Reactivity Note:
Demonstrated to react with human and rat.
Application Notes:
Western Blot Analysis: 1 µg/mL from a previous lot detected acetyl CoA Carboxylase on 10 µg of rat heart lysate.
Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected acetyl CoA Carboxylase on 10 µg of human heart lysate.
Control:
Human heart lysate
Quality Assurance:
Evaluated by Western Blot in human heart lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected acetyl CoA Carboxylase on 10 µg of human heart lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q13085
Entrez Gene Number:
NP_942131
Gene Symbol:
ACAC
ACC
ACC-alpha
ACC1
ACCA
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Alternate Names:
acetyl-CoA carboxylase 1
acetyl-CoA carboxylase-alpha
acetyl-Coenzyme A carboxylase alpha
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Acetyl-CoA carboxylase (ACC) is a complex multifunction |