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Anti-Phosphotyrosine, 4G10® Platinum
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REFERENCES
Pro-neural miR-128 is a glioma tumor suppressor that targets mitogenic kinases.
T Papagiannakopoulos,D Friedmann-Morvinski,P Neveu,J C Dugas,R M Gill,E Huillard,C Liu,H Zong,D H Rowitch,B A Barres,I M Verma,K S Kosik (2012) Oncogene.31
The metastasis gene NEDD9 product acts through integrin β3 and Src to promote mesenchymal motility and inhibit amoeboid motility.
Jessica Ahn,Victoria Sanz-Moreno,Christopher J Marshall (2012) Journal of cell science.125
Prolactin-induced activation of phagocyte NADPH oxidase in the teleost fish gilthead seabream involves the phosphorylation of p47phox by protein kinase C.
Víctor H Olavarría,Jaime E Figueroa,Victoriano Mulero (2012) Developmental and comparative immunology.36
Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBP? expression and contributes to transformation of breast tumor cells.
Tapasree Roysarkar,Shikha Sharan,Jun Wang,Snehalata A Pawar,Carrie A Cantwell,Peter F Johnson,Deborah K Morrison,Ju-Ming Wang,Esta Sterneck,Tapasree Roy Sarkar,Tapasree Roy Sarkar (2012) Molecular and cellular biology.32
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Species Reactivity Key Applications Host Format Antibody Type
A WB, ICC, ELISA, IHC, IF, FC, IP Mouse Purified Monoclonal Antibody
Description:
4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)
Promotional Text:
FREE Positive Control Included:
EGF-stimulated A431 cell lysate (cat. # 12-302)
Special Offer on Antibodies! Click Here!
Trade Name:
Upstate (Millipore)
Specificity:
Tyrosine-phosphorylated proteins from all species
Molecular Weight:
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Immunogen:
4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein
Modifications:
Phosphorylation
Isotype:
IgG2b
Background Information:
The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of.
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Species Reactivity:
All
Application Notes:
Immunoprecipitation: 2-4 μL of a previous lot can immunoprecipitate quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.
Control:
Suggested Positive Antigen Control: Cat. # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20ºC. The lysate is stable for 6 months at -20ºC. Before use, add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
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