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Anti-Met (extracellular) Antibody, clone 4F8.2
Recommended Replacement for: 05-237
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REFERENCES
Cellular mechanisms underlying the regulation of dendritic development by hepatocyte growth factor.
Charles Finsterwald,Jean-Luc Martin (2011) The European journal of neuroscience.34
Crystal structure of the tyrosine kinase domain of the hepatocyte growth factor receptor c-Met and its complex with the microbial alkaloid K-252a.
Schiering, Nikolaus, et al. (2003) Proc. Natl. Acad. Sci. U.S.A., 100: 12654-9 (2003)
Species Reactivity Key Applications Host Format Antibody Type
H IH(P), WB Mouse Purified Monoclonal Antibody
Description:
Anti-Met (extracellular) Antibody, clone 4F8.2
Replaces:
05-237
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Specificity:
Detects Met on its extracellular domain.
Molecular Weight:
170 kDa
Epitope:
Extracelluar domain (N-term)
Immunogen:
GST-fusion protein corresponding to amino acids 80-434 of human Met.
Clone:
4F8.2
Isotype:
IgG2bκ
Background Information:
The proto-oncogene Met (c-Met, HGF/SF Receptor, HGFR) is a receptor tyrosine kinase that is activated upon Hepatocyte Growth Factor (HGF) binding. This stimulates the autophosphorylation on Tyr1234/Tyr1235 which leads to pleiotropic downstream signaling events in epithelial cells that stimulate multiple cellular processes including cell proliferation, growth, differentiation, cell migration/ invasion, and tumorigenesis. These effects are mediated by SH2 domain-containing proteins that bind to the C-terminal tail of c-Met. Chronic stimulation of Met on cancer cells is thought to play a role in metastasis and dysregulation of c-Met activity is directly associated with oncogenesis, making it an important cancer target.
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Species Reactivity:
Human
Species Reactivity Note:
Human
Application Notes:
Western Blot Analysis:
This lot detected Met at 1:1,000 dilution in HEK293 cell lysate resolved via SDS-PAGE and transferred to PVDF.
Immunohistochemistry Analysis :
Quality Assurance:
Routinely evaluated by Western Blot.
Western Blot Analysis:
This lot detected Met at 1:1,000 dilution in HEK293 cell lysate resolved via SDS-PAGE and transferred to PVDF.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% NaN3.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
UniProt Number:
P08581
Entrez Gene Number:
NP_001120972.1
Gene Symbol:
AUTS9
EC 2.7.10.1
HGFR
OTTHUMP00000069168
RCCP2
c-Met
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Alternate Names:
HGF receptor
HGF/SF receptor
Met proto-oncogene tyrosine kinase
SF receptor
Scatter factor receptor
met proto-oncogene
met proto-oncogene (hepatocyte growth factor receptor)
oncogene MET
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunohistochemistry (Paraffin)
Western Blotting
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