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Anti-phospho-EGFR (Tyr1173), clone 3G3.2
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PRODUCT FAMILY INFORMATION
EGFR Antibodies, Kits & Proteins
Millipore’s EGFR (Epidermal growth factor receptor) antibodies, kits, and proteins are high quality, validated tools for EGFR research. See below for a selection of EGFR and phospho-EGFR products.
Millipore’s EGFR antibodies, kits and proteins are high quality, validated tools for EGFR research. See below for data and references for Millipore’s EGFR products. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Species Reactivity Key Applications Host Format Antibody Type
H WB, FC, IHC, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2
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Specificity:
This antibody recognizes phosphorylated EGFR (Tyr1173).
Molecular Weight:
175 kDa
Epitope:
Phosphorylated Tyr1173
Immunogen:
KLH-conjugated linear peptide corresponding to residues surrounding Tyr1173 of EGFR.
Modifications:
Phosphorylation
Clone:
3G3.2
Isotype:
IgG1κ
Background Information:
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of five tyrosine (Y) residues in the C-terminal domain of EGFR occurs.
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Species Reactivity:
Human
Species Reactivity Note:
Proven to react with human.
Application Notes:
Flow Cytometry Analysis: A431 cells stained using 10 ug/mL of anti-phospho -EGFR (Tyr1173).
Immunocytochemistry Analysis: A 1:500 dilution of this antibody
detected anti-phospho-EGFR (Tyr1173) in EGF treated A431
lysates.
Immunohistochemistry Analysis: A 1:100 dilution of this detected anti-phospho-EGFR (Tyr1173) in serous ovarian cancer.
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Control:
EGF treated A431 cell lysate
Quality Assurance:
Evaluated by Western Blot in EGF treated and untreated A431 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected phospho-EGFR (Tyr1173) on 15 µg of EGF treated A431 cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified rabbit ascites containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.
Storage Conditions:
Maintain refrigerated at 2-8°C for 1 year from date of receipt.
UniProt Number:
Q86T13
Entrez Gene Number:
NP_005219.2
Gene Symbol:
C14orf27
CEG1
EGFR-5
EGFR5
OTTHUMP00000178873
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Alternate Names:
C-type lectin domain family 14, member A
ClECT and EGF-like domain containing protein
Epidermal growth factor receptor 5
chromosome 14 open reading frame 27
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our pro |