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Anti-phospho-Akt (Ser473), clone 6F5
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PRODUCT FAMILY INFORMATION
Phospho-Akt Antibodies, Kits & Assays
Millipore’s phosphoAkt antibodies have been well validated and published. See below or a comprehensive list of our phosphoAkt products, based on the expertise of Upstate & Chemicon.
EMD Millipore’s phosphoAkt antibodies are based on the expertise of Upstate & Chemicon. Our phosphoAkt antibodies have been well validated and published. See the ordering tab for a comprehensive list of our phosphoAkt products and direct links to their technical pages for data, references and related products.
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Vrt ELISA, WB, FC, IF, IHC Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-Akt (Ser473) Antibody, clone 6F5
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Specificity:
Recognizes Akt only when phosphorylated on Ser473. This antibody does not cross-react with Akt1/PKBα when it is not phosphorylated on Ser473.
Molecular Weight:
60 kDa
Epitope:
Phosphorylated Ser473
Immunogen:
Peptide encompassing and including phosphorylated Ser473 of human Akt1.
Modifications:
Phosphorylation
Clone:
6F5
Isotype:
IgG1κ
Background Information:
Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved in multiple signaling pathways that relate to many biological processes including glucose metabolism, cell survival/apoptosis, cell cycle control, angiogenesis, differentiation, and cell growth and proliferation. In mammals three isoforms of Akt (Akt1/PKB α, Akt2/PKBβ, and Akt3/PKBγ) exists. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt1 is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development, and adipocyte differentiation. Akt2 is correlated with the regulation of glucose homeostasis and is the predominant isoform expressed in insulin-responsive tissues. Akt3 is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal Pleckstrin Homology (PH) domain that provides a lipid-binding module to direct Akt to PIP3 at the cell membrane that is necessary for its activation, a central catalytic domain containing Thr308, and a C-terminal hydrophobic motif containing Ser473. The activation of Akt is dependent on a dual regulatory mechanism that requires both its translocation to the plasma membrane and dual phosphorylation on Thr308 and Ser473 by PDK1 and the TORC2 complex, respectively.
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Species Reactivity:
Human
Mouse
Rat
Vertebrates
Species Reactivity Note:
Human, mouse, and rat tested. Expected to react with most species as sequence is 100% homologous with most common vertebrate.
Quality Assurance:
Western Blot Analysis: 0.5-1 μg/mL of this antibody detected phosphorylated Akt in lysates from mouse NIH-3T3 lysate treated with insulin.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monolconal IgG1 in PBS, no preservative.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P31749
Entrez Gene Number:
NP_001014431
Gene Symbol:
AKT
C-AKT
EC 2.7.11.1
MGC99656
PKB
PKB-ALPHA
PRKBA
RAC
RAC-ALPHA
RAC-PK-alpha
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Alternate Names:
Protein kinase B
RAC-alpha serine |