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Anti-FOXP3, clone 3G3
Species Reactivity Key Applications Host Format Antibody Type
M, H FC, ELISA Mouse Purified Monoclonal Antibody
Description:
Anti-FOXP3 Antibody, clone 3G3
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Specificity:
This antibody recognizes the N-terminus of Forkhead box protein P3 (FOXP3).
Molecular Weight:
Expected molecular weight ~47 kDa.
On western blot, a molecular weight of ~49-55 kDa may be observed (Bolzer, K., et al., 2009).
Epitope:
N-terminus
Immunogen:
Histadine-tagged recombinant protein corresponding to the N-terminus of mouse FOXP3.
Isotype:
IgG1κ
Background Information:
The regulation of tolerance to self-antigens is a necessary biological process in order to prevent autoimmunity. CD4+ CD25+ regulatory T cells are known regulators of immune tolerance and activation. In both human and mice species, CD4+ CD25+ regulatory T cells are consistent in their expression of the transcription factor FOXP3 (forkhead box P3). FOXP3 acts as the “master switch” for the function and development of regulatory T cells. Mutations and deletions in the sequence encoding for FOXP3 expression lead to an inability to express FOXP3, causing an inability to regulate T cell development and leading to fatal autoimmune diseases such as IPEX and XLAAD. Conversely, recent studies have shown high and wide expression of FOXP3 in several cancer cell lines and tumors, indicating that FOXP3 is involved in preventing immunity against malignant tumors.
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Species Reactivity:
Mouse
Human
Species Reactivity Note:
Demonstrated to react with human and mouse.
Application Notes:
Flow Cytometry Analysis: 1 µg from a previous lot of a biotinylated version of this antibody detected FOXP3 in human PBMC's. THIS ANTIBODY IS NOT BIOTINYLATED.
ELISA: A previous lot of this antibody was shown to work in ELISA by an independent laboratory (Gavin, M., et al., 2006).
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Control:
Mouse CD4+ CD25+ splenocytes
Quality Assurance:
Evaluated by Flow Cytometry in mouse CD4+ CD25+ splenocytes.
Flow Cytometry Analysis: 5 µg of a biotinylated version of this antibody detected FOXP3 in mouse CD4+ CD25+ splenocytes.
THIS ANTIBODY IS NOT BIOTINYLATED.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q99JB6
Entrez Gene Number:
NP_473380
Gene Symbol:
FOXP3
AIID
DIETER
IPEX
JM2
PIDX
SCURFIN
Scurfin
XPID
scurfin
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Alternate Names:
forkhead box P3
immune dysregulation, polyendocrinopathy, enteropathy, X-linked
immunodeficiency, polyendocrinopathy, enteropathy, X-linked
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
ELISA
Entrez Gene Summary:
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocri |