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Anti-JNK3/SAPK1b, clone C05T
Recommended Replacement for: 05-893
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REFERENCES
SUMOylation of the kainate receptor subunit GluK2 contributes to the activation of the MLK3-JNK3 pathway following kainate stimulation.
Qiu-Ju Zhu,Yan Xu,Cai-Ping Du,Xiao-Yu Hou (2012) FEBS letters.586
Species Reactivity Key Applications Host Format Antibody Type
H, R WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-JNK3/SAPK1b Antibody, clone C05T, rabbit monoclonal
Replaces:
05-893
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Specificity:
Recognizes JNK3/SAPK1b. Does not cross react with JNK1 or JNK2.
Molecular Weight:
46-54kDa
Immunogen:
GST fusion protein corresponding to full-length rat JNK3/ SAPK1b
Clone:
C05T
Isotype:
IgG
Background Information:
The stress-activated protein kinase 1 family is also referred to as the jun N-terminal kinase family, in light of the substrate preference of these serine/threonine kinases. The SAPK family shares 42-45% identity in the kinase domains with the MAPK family, and the SAPKs are activated by phosphorylation of a threonine and tyrosine by MKK4 and SKK4, just as the MAP kinases are phosphorylated on a threonine and tyrosine by MEK1 and MEK2. By contrast to the mitogen-activated kinases, the stress-activated kinases are only weakly activated by mitogenic stimuli, but potently activated by stress stimuli, such as inflammatory cytokines, ischemia, chemotherapeutic agents and irradiation.
The JNK/SAPK1 kinases, like the other MAPK-like kinases, are thought to phosphorylate multiple substrates and regulate many processes, including proliferation (in some cell types) and apoptosis. The SAPK2/3 family is most widely referred to as the p38 family. These kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. The activating kinases of SAPK2/3 are SKK2/MEK3 for SAPK2a and 2b, and MKK6 for SAPK3. The targets of the SAPK2/3 family include the MAPKAP kinases 2 and 3/3pK. In addition, SKK4 is related to this family, exhibiting 60% identity, and is activated by MKK6.
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Species Reactivity:
Human
Rat
Application Notes:
Western Blot Analysis: A 1:500-1:2,000 dilution of this lot detected JNK3 in RIPA lysates from A431 cells.
Control:
RIPA cell lysates from A431 cells.
Quality Assurance:
Routinely evaluated by western blot on RIPA lysates from A431 cells.
Presentation:
Cultured supernantant in 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
UniProt Number:
P49187
Entrez Gene Number:
NM_002753.2
Gene Symbol:
EC 2.7.11.24
FLJ12099
FLJ33785
JNK3
JNK3A
MGC50974
OTTHUMP00000161180
OTTHUMP00000161182
OTTHUMP00000161183
PRKM10
p493F12
p54bSAPK
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Alternate Names:
JNK3 alpha protein kinase
MAP kinase
MAP kinase p49 3F12
Stress-activated protein kinase JNK3
c-Jun N-terminal kinase 3
c-Jun kinase 3
mitogen-activated protein kinase 10
stress activated protein kinase JNK3
stress activated protein kinase beta
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumptio |