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Anti-Rho (-A, -B, -C), clone 3L74
Recommended Replacement for: 05-822
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REFERENCES
Prognostic value of rho GTPases and rho guanine nucleotide dissociation inhibitors in human breast cancers.
Jiang, Wen G, et al. (2003) Clin. Cancer Res., 9: 6432-40 (2003)
The PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization.
Vincent, S and Settleman, J (1997) Mol. Cell. Biol., 17: 2247-56 (1997)
Signal-transducing protein phosphorylation cascades mediated by Ras/Rho proteins in the mammalian cell: the potential for multiplex signalling.
Denhardt, D T (1996) Biochem. J., 318 ( Pt 3): 729-47 (1996)
Species Reactivity Key Applications Host Format Antibody Type
R, H WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal
Replaces:
05-822
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Rho (-A, -B, -C)
Molecular Weight:
Mr 22kDa
Immunogen:
KLH-conjugated, synthetic peptide (SYPDTDVILMCFSIDSPDSLENKK) corresponding to amino acids 73-94 of human Rho (-A, -B, -C), with two terminal lysines added to increase peptide solubility
Clone:
3L74
Isotype:
IgG
Background Information:
Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganisation, and membrane trafficking.
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Species Reactivity:
Rat
Human
Species Reactivity Note:
Broad species cross-reactivity expected based on sequence homology
Application Notes:
Western Blot Analysis:
A 1:1000-1:4000 dilution of this lot detected Rho (-A, -B, -C) in RIPA lysates from human HL-60 and rat PC-12 cells. This antibody also recognized recombinant RhoA protein, but not recombinant rac1 or cdc42 proteins.
Quality Assurance:
routinely evaluated by immunoblot on modified RIPA lysates from HL-60 cells
Presentation:
Cultured supernantant in 0.05% sodium azide
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P61586
Entrez Gene Number:
NM_001664
Gene Symbol:
ARH12
ARHA
H12
RHOH12
RhoA
Rho12
RHO12
RHOA
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Alternate Names:
ras homolog
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P61586 # Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosi |