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Anti-phospho-Ribosomal Protein S6 (Ser235), clone MC27
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-phospho-Ribosomal Protein S6 (Ser235) Antibody, clone MC27, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes ribosomal protein S6 only when phosphorylated on Ser235.
Molecular Weight:
~30 kDa
Epitope:
Phosphorylated Ser235
Immunogen:
Synthetic peptide containing the sequence …RRL[pS]SL… in which pS corresponds to phospho-serine at residue 235 of human ribosomal protein S6. The immunizing sequence is identical in mouse, rat, chicken and Xenopus.
Modifications:
Phosphorylation
Clone:
MC27
Isotype:
IgG
Background Information:
S6 (Ribosomal protein S6, RpS6, 40S ribosomal protein S6) is known to play a role in cell growth and proliferation. It lays downstream of both the PI3K/Akt/mTOR pathway as a substrate of p70 S6 kinase and the Ras/Erk pathway as a substrate of RSK. S6 is phosphorylated in response to stimulation by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. There are two major areas of S6 phosphorylation; Ser235/Ser236 that is known to be phosphorylated by both p70S6 Kinase as well as RSK as well as Ser240/244 which is thought to only be phosphorylated by p70S6K.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, rat and mouse. Broad species cross-reactivity predicted, due to sequence homology.
Application Notes:
Western Blot Analysis:
A 1:500-1:2,000 dilution of this lot detected phosphorylated ribosomal protein S6 in lysates from serum stimulated 3T3 cells.
Control:
Serum stimulated 3T3 cell lysate.
Quality Assurance:
Routinely evaluated by western blot on serum stimulated 3T3 cell lysate.
Presentation:
Cultured supernatant with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P62753
Entrez Gene Number:
NM_001010.2
Gene Symbol:
40S ribosomal protein S6
Phosphoprotein NP33
ribosomal protein S6
RPS6
Alternate Names:
40S ribosomal protein S6
Phosphoprotein NP33
ribosomal protein S6
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phos |