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Anti-phospho-CENP-A (Ser7), clone NL41
Recommended Replacement for: Cat. # 05-792. 05-792 will only be availabe on a custom basis.
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REFERENCES
Cdc48/p97-Ufd1-Npl4 antagonizes Aurora B during chromosome segregation in HeLa cells.
Grzegorz Dobrynin,Oliver Popp,Tina Romer,Sebastian Bremer,Michael H A Schmitz,Daniel W Gerlich,Hemmo Meyer (2011) Journal of cell science.124
Species Reactivity Key Applications Host Format Antibody Type
H Mplex, WB, ICC Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-phospho-CENP-A (Ser7) Antibody, clone NL41, rabbit monoclonal
Replaces:
Cat. # 05-792. 05-792 will only be availabe on a custom basis.
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Trade Name:
Upstate (Millipore)
Product Overview:
CENP-A is a variant version of histone H3 found only at centromeres. It is phosphorylated at serine 7 during mitosis.
Specificity:
CENP-A phosphorylated on serine 7
Molecular Weight:
~17kDa
Immunogen:
peptide containing the sequence RRpSRK in which pS is phospho-serine corresponding to amino acid 7 of human CENP-A (centromere protein A)
Modifications:
Phosphorylation
Clone:
clone NL41
Isotype:
IgG
Background Information:
Centromere protein A (CENP-A) is a 17 kDa centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of α-satellite DNA into primary chromation structure. CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker.
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Species Reactivity:
Human
Species Reactivity Note:
Human. Others not tested.
Control:
Acid extracted proteins from colcemid-arrested HeLa cells
Quality Assurance:
routinely evaluated by immunoblot on acid extracted proteins from colcemid-arrested HeLa cells (Catalog #17-306)
Presentation:
Cultured supernantant in 0.05% sodium azide
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P49450
Entrez Gene Number:
NM_001809
Gene Symbol:
CENPA
CENP-A
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Multiplexing
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distin |