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Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304
Recommended Replacement for: 05-791
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REFERENCES
The development of IL-17/IFN-γ-double producing CTLs from Tc17 cells is driven by epigenetic suppression of Socs3 gene promoter.
Takayuki Satoh,Masaki Tajima,Daiko Wakita,Hidemitsu Kitamura,Takashi Nishimura (2012) European journal of immunology.42
Progressive histone alterations and proinflammatory gene activation: consequences of heme protein/iron-mediated proximal tubule injury.
Zager RA, Johnson AC (2010) American journal of physiology. Renal physiology.298:F827-37. Epub 2009 Dec 23.
Histone H3K4 demethylases are essential in development and differentiation.
Benevolenskaya, Elizaveta V (2007) Biochem. Cell Biol., 85: 435-43 (2007)
Methylation of histone H3 at lysine 4 is highly conserved and correlates with transcriptionally active nuclei in Tetrahymena.
Strahl, B D, et al. (1999) Proc. Natl. Acad. Sci. U.S.A., 96: 14967-72 (1999)
Species Reactivity Key Applications Host Format Antibody Type
H, Vrt WB, PIA, Mplex Rabbit null Monoclonal Antibody
Description:
Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304 | 04-791
Replaces:
05-791
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Trade Name:
Upstate (Millipore)
Specificity:
Monomethyl, dimethyl, and trimethyl histone H3 (Lys4). Additional unidentified bands above 50 kDa are detected in some samples
Molecular Weight:
~17 kDa
Epitope:
Lys4 of Histone H3
Immunogen:
KLH-conjugated, synthetic peptide containing …Tme2KQT… in which me2K corresponds to dimethyl-lysine at residue 4 of human histone H3.
Modifications:
Methylation
Clone:
AW304
Isotype:
IgG
Background Information:
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Histone methylation is primarily associated with heterochromatin and gene silencingView All »
Species Reactivity:
Human
Vertebrates
Species Reactivity Note:
Human. The immunizing sequence is conserved from Tetrahymena to human, so broad cross-reactivity is expected.
Application Notes:
Chromatin Immunoprecipitation:
The antibody was reported by an independent laboratory as suitable for ChIP.
Immunoblot Analysis:
A previous lot detected methylated Histone H3 in acid-extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant histone H3 (Catalog # 14-494).
Peptide Inhibition Analysis:
2 μM of a histone H3 peptide containing monomethyl-lysine 4, dimethyl lysine 4, and trimethyl-lysine 4 abolished detection of histone H3 by this lot in immunoblot analysis of HeLa acid extracts. Some signal reduction was observed with histone H3 peptides containing dimethyl-lysine 9 or 27.
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