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Anti-monomethyl-Histone H4 (Lys20), clone NL314
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REFERENCES
Histone H4 lysine 20 of Saccharomyces cerevisiae is monomethylated and functions in subtelomeric silencing.
Christopher R Edwards,Weiwei Dang,Shelley L Berger (2011) Biochemistry.50
L3MBTL1, a histone-methylation-dependent chromatin lock.
Trojer, Patrick, et al. (2007) Cell, 129: 915-28 (2007)
A chromosomal memory triggered by Xist regulates histone methylation in X inactivation.
Alexander Kohlmaier, Fabio Savarese, Monika Lachner, Joost Martens, Thomas Jenuwein, Anton Wutz (2004) PLoS biology.2:E171
A trans-tail histone code defined by monomethylated H4 Lys-20 and H3 Lys-9 demarcates distinct regions of silent chromatin.
Sims, Jennifer K, et al. (2006) J. Biol. Chem., 281: 12760-6 (2006)
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Species Reactivity Key Applications Host Format Antibody Type
H WB, Mplex Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes monomethyl-histone H4 (Lys20), Mr ~10kDa.
Molecular Weight:
10kDa
Immunogen:
KLH-conjugated, synthetic peptide containing the sequence …HR[mmK]V…, in which mmK corresponds to monomethyl-lysine at residue 20 of human histone H4.
Modifications:
Methylation
Clone:
NL314
Isotype:
IgG
Background Information:
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Trimethylation of histone H4 on lys20 (H4-K20me3) selectively marks constitutive pericentromeric hetero-chromatin and imprinting control regions, while mono- and dimethylation are broadly distributed but principally enriched in euchromatin regions. Although the biological function of H4K20me remains poorly understood, studies suggest that H4-K20me2 is involved in the guidance of DNA repair proteins to DNA strand breaks, whereas H4K20me1 is associated with chromatin condensation processes. PR-Set7 (also known as SET8) specifically catalyzes monomethylation of H4K20me1.
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Species Reactivity:
Human
Species Reactivity Note:
Human. The immunizing sequence is conserved from Tetrahymena to human, so broad species cross-reactivity is expected.
Application Notes:
Immunoblot Analysis:
A 1:4000 dilution of a previous lot detected methylated histone H3 in acid extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant Histone H3.
Multiplexing:
Beadlyte® Histone-Peptide Specificity Assay: 1:2,000-1:30,000 dilutions of a previous lot were incubated with histone H4 peptides containing various modifications conjugated to Luminex® microspheres. Specificity to monomethyl-histone H4 (Lys20) was demonstrated (Figure C).
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Control:
HeLa acid extracts
Quality Assurance:
Routinely evaluated by immunoblot analysis.
Immunoblot Analysis:
A 1:1000-1:2000 dilution of this lot detected monomethyl histone H4 (Lys20) in HeLa acid extracts. (Figure A).
Presentation:
Cultured supernantant in 0.05% sodium azide
Storage Conditions:
St |