DICER, Ms x 5D12.2-100ug

Anti-Dicer1, clone 5D12.2 Antibody
Species Reactivity Key Applications Host Format Antibody Type
H  WB Mouse Purified Monoclonal Antibody
Description:
Anti-Dicer1 Antibody, clone 5D12.2
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Trade Name:
Upstate (Millipore)
Specificity:
Human Dicer1
Molecular Weight:
220 kDa
Immunogen:
KLH conjugated synthetic peptide corresponding to amino acids 378-385 of human Dicer1
Clone:
5D12.2
Isotype:
IgG1κ
Background Information:
RNA interference (RNAi) is mechanism for post-transcriptional gene silencing that leads to the loss of the activity of the gene. RNAi is activated when long dsRNAs are cleaved by the enzyme Dicer1 (RNase III family of dsRNA-specific endonucleases) into 21-23 nucleotide siRNA (small interfering RNA). These siRNAs bind proteins to form the RISC (RNA-induced silencing complex) which is activated when the double stranded siRNAs are unzipped to single stranded siRNAs. Activated RISC recognizes and binds complementary sequences in mRNA. The mRNA is cleaved and the overall effect is an inhibition of the protein expression and a loss of the gene activity. Dicer1 along with the Argonaute family of proteins (Ago1 and Ago2) share the same domain PIWI and PAZ domain that provide endonuclease activity to RNA-induced silencing complexes (RISC).
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Species Reactivity:
Human
Application Notes:
Western blot
Quality Assurance:
Routinely evaluated by western blot on lysates from HeLa nuclear extracts.
Purification Method:
Protein G chromatography
Storage Conditions:
Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
UniProt Number:
Q9UPY3
Entrez Gene Number:
NP_085124.2
Gene Symbol:
DICER1
DICER
HERNA
Alternate Names:
DCR1
Dicer
HERNA
KIAA0928
Key Applications:
Western Blotting
Brand Family:
Upstate
Product Name:
Anti-Dicer1, clone 5D12.2 Antibody
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μL
Format:
Purified
Host:
Mouse
Applications:
Anti-Dicer1 Antibody, clone 5D12.2 is a Mouse Monoclonal Antibody for detection of Dicer1 also known as DCR1, Dicer, HERNA, KIAA0928 & has been validated in WB.