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Anti-Caspase-8 Antibody, rabbit monoclonal
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REFERENCES
Velogenic newcastle disease virus as an oncolytic virotherapeutics: in vitro characterization.
Rajiv Kumar,Ashok K Tiwari,Uttara Chaturvedi,G Ravi Kumar,Aditya P Sahoo,R S Rajmani,Lovleen Saxena,Shikha Saxena,Sangeeta Tiwari,Sudesh Kumar (2012) Applied biochemistry and biotechnology.167
Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death.
Boldin, M P, et al. (1996) Cell, 85: 803-15 (1996)
Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases
Srinivasula, S M, et al (1996) Proc Natl Acad Sci USA, 93:14486-91 (1996)
FLICE is activated by association with the CD95 death-inducing signaling complex (DISC)
Medema, J P, et al (1997) EMBO J, 16:2794-804 (1997)
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Species Reactivity Key Applications Host Format Antibody Type
H, R IP, WB, FC Rabbit Purified Monoclonal Antibody
Description:
Anti-Caspase-8 Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Rat Caspase-8.
Immunogen:
A synthetic peptide corresponding to N-terminal residues of human Caspase-8 subunit p18. The antibody should recognize both pro-form (55kDa) and p18 cleaved-form of Caspase-8.
Clone:
E7
Background Information:
Caspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death (1-4). Caspase-8 is recruited to death-inducing signaling complex (DISC) CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18 (1-4). Active caspase-8, the first in the caspase signaling cascade, signals downstream caspases to eventually induce apoptosis.
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Species Reactivity:
Human
Rat
Species Reactivity Note:
Human, Rat (positive control: Hela cell lysate)
Application Notes:
WB: 1:500
Flow Cytometry: 1:20
IP: 1:40
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
Q14790
Entrez Gene Number:
NM_001080124.1
NM_001080125.1
NM_001228.4
NM_033355.3
NM_033356.3
NM_033358.3
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Gene Symbol:
CASP8
MCH5
MGC78473
CASP-8
MACH
ALPS2B
FLICE
procaspase-8
CAP4
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Flow Cytometry
Entrez Gene Summary:
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires |