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Anti-Caspase 2 Antibody, rabbit monoclonal
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REFERENCES
Activation of caspase-2 in apoptosis
Li, H, et al (1997) J Biol Chem, 272:21010-7 (1997)
Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death
Wang, L, et al (1994) Cell, 78:739-50 (1994)
Apoptosis signaling triggered by the marine alkaloid ascididemin is routed via caspase-2 and JNK to mitochondria
Dirsch, Verena M, et al (2004) Oncogene, 23:1586-93 (2004)
Caspase-2-induced apoptosis is dependent on caspase-9, but its processing during UV- or tumor necrosis factor-dependent cell death requires caspase-3
Paroni, G, et al (2001) J Biol Chem, 276:21907-15 (2001)
Species Reactivity Key Applications Host Format Antibody Type
H, M FC, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-Caspase 2 Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse Caspase-2.
Immunogen:
A synthetic peptide corresponding to N-terminus of human Caspase-2 p15 subunit. The antibody should recognize both pro-form (~50kDa) and p15 cleaved-form of Caspase-2.
Clone:
Y61
Background Information:
Caspase-2 (Nedd2/ICH-1) is part of a family of aspartate-specific cysteine proteases that participate in programmed cell death, or apoptosis. Caspase-2 is localized to the nucleus, and has been shown to play roles in both positive and negative regulation of apoptosis (1). Upon apoptotic stimulation, pro-caspase-9 is cleaved in a series of steps to produce an 18-kDa large subunit and a 12-kDa small fragment (2). Caspase-2 can be a nuclear initiator caspase, as it has been shown to precede the processing and activation of caspase-8, -9, and -3 (3). Caspase-2 might also act as an effector caspase; in apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of caspase-9 and caspase-3 activation (4).
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human, Mouse (positive control: Jurkat cell lysate)
Application Notes:
WB: 1:500
Flow Cytometry: 1:10
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P42575
Entrez Gene Number:
NM_032982.2
NM_032983.2
Gene Symbol:
CASP2
NEDD2
ICH1
ICH-1L/1S
ICH-1L
NEDD-2
CASP-2
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Western Blotting
Entrez Gene Summary:
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript var |