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Anti-MAPKAP-K2 Antibody, clone 7H4.2
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REFERENCES
MAPK-activated protein kinase 2 deficiency in microglia inhibits pro-inflammatory mediator release and resultant neurotoxicity. Relevance to neuroinflammation in a transgenic mouse model of Alzheimer disease.
Culbert, Ainsley A, et al. (2006) J. Biol. Chem., 281: 23658-67 (2006)
MAPKAPK2 and HSP27 are downstream effectors of p38 MAP kinase-mediated matrix metalloproteinase type 2 activation and cell invasion in human prostate cancer.
Xu, L, et al. (2006) Oncogene, 25: 2987-98 (2006)
MAPKAP kinase-2 is a cell cycle checkpoint kinase that regulates the G2/M transition and S phase progression in response to UV irradiation.
Manke, Isaac A, et al. (2005) Mol. Cell, 17: 37-48 (2005)
CDC25B phosphorylation by p38 and MK-2
Lemaire, Matthieu, et al (2006) Cell Cycle, 5:1649-53 (2006)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-MAPKAP-K2 Antibody, clone 7H4.2
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Trade Name:
Upstate (Millipore)
Molecular Weight:
45 kDa
Immunogen:
KLH-conjugated synthetic peptide of the C-terminus of the long form of human MAPKAP-K2.
Clone:
7H4.2
Isotype:
IgG
Species Reactivity:
Human
Mouse
Rat
Application Notes:
Suggested dilutions:
Immunoblot Analysis: 1:500
Immunoprecipitation: 1:20
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Protein A purfied
Presentation:
200 µg (1 mg/mL) of mouse monoclonal IgG in PBS containing 0.1% sodium azide.
Storage Conditions:
2 years at -20°C from date of shipment.
UniProt Number:
P49137
Entrez Gene Number:
NM_004759.3
NM_032960.2
Gene Symbol:
MAPKAPK2
MK2
MAPKAPK-2
Alternate Names:
MAPK-activated protein kinase 2, MAPKAP kinase 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
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UniProt Summary:
FUNCTION: SwissProt: P49137 # Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
SIZE: 400 amino acids; 45568 Da
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