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Anti-Bcl-2 Antibody, rabbit monoclonal
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REFERENCES
Autophagy is involved in traumatic brain injury-induced cell death and contributes to functional outcome deficits in mice.
C-L Luo,B-X Li,Q-Q Li,X-P Chen,Y-X Sun,H-J Bao,D-K Dai,Y-W Shen,H-F Xu,H Ni,L Wan,Z-H Qin,L-Y Tao,Z-Q Zhao (2011) Neuroscience.184
Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death
Hockenbery, D, et al (1990) Nature, 348:334-6 (1990)
Phosphorylation of Bcl2 and regulation of apoptosis
Ruvolo, P P, et al (2001) Leukemia, 15:515-22 (2001)
Species Reactivity Key Applications Host Format Antibody Type
H, M FC, IF, IP, WB, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Bcl-2 Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes a region between residues BH3 and BH4 of human Bcl-2.
Molecular Weight:
26 kDa
Immunogen:
KLH-conjugated synthetic peptide between residues BH3 and BH4 of human Bcl-2.
Isotype:
IgG
Species Reactivity:
Human
Mouse
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Storage Conditions:
2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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UniProt Number:
P10415
Entrez Gene Number:
NM_000633.2
NM_000657.2
Gene Symbol:
BCL2
Bcl-2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunofluorescence
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
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Entrez Gene Summary:
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
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UniProt Summary:
FUNCTION: SwissProt: P10415 # Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
SIZE: 239 amino acids; 26266 Da
SUBUNIT: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L |