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Anti-PI3 Kinase Antibody, p110β, rabbit monoclonal
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REFERENCES
A function for phosphoinositide 3-kinase beta lipid products in coupling beta gamma to Ras activation in response to lysophosphatidic acid
Yart, Armelle, et al (2002) J Biol Chem, 277:21167-78 (2002)
Increased insulin sensitivity in mice lacking p85beta subunit of phosphoinositide 3-kinase
Ueki, Kohjiro, et al (2002) Proc Natl Acad Sci USA, 99:419-24 (2002)
Species Reactivity Key Applications Host Format Antibody Type
H FC, IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-PI3 Kinase Antibody, p110β, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes a region in the N-terminus domain of human PI3 Kinase, p110β. Non specific band detected at MW 55 kDa.
Molecular Weight:
110 kDa
Epitope:
N-terminus
Immunogen:
KLH-conjugated synthetic peptide corresponding to the N-terminal region of human PI3 Kinase, p110β
Clone:
Y384
Isotype:
IgG
Background Information:
PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
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Species Reactivity:
Human
Species Reactivity Note:
Proven to react with human. Reactivity with other species has not been confirmed.
Application Notes:
Immunoprecipitation:
A 1:50 dilution of a previous lot of this antibody immunoprecipitated PI3 Kinase, p110β using protein G:agarose beads.
Flow Cytometry:
A 1:40 of a previous lot of this antibody stained cells for intracellular staining for flow Cytometry analysis.
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Control:
Jurkat cell lysate
Quality Assurance:
Routinely evaluated by Western Blot on jurkat cell lysate.
Western Blot Analysis:
A 1:500 dilution of this lot detected endogenous PI3 Kinase, p110β in lysate from Jurkat cells.
Purification Method:
Protein A purfied
Presentation:
Purified rabbit monoclonal IgG in buffer containing 0.03% BSA, 30 mM Tris-Glycine (pH 7.4), 0.09 M NaCl, 0.006% sodium azide as a preservative in a solution of 40% glycerol
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage
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UniProt Number:
P42338
Entrez Gene Number:
NM_006219.1
Gene Symbol:
PIK3CB
PI3Kbeta
MGC133043
PIK3C1
DKFZp779K1237
Alternate Names:
PI3-kinase p110 subunit beta
PtdIns-3-kinase p110
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