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Anti-PI3 Kinase Antibody, p110α rabbit monoclonal
Species Reactivity Key Applications Host Format Antibody Type
H IF, IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-PI3 Kinase Antibody, p110α rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes the C-terminal domain of PI3 Kinase, p110α.
Molecular Weight:
110 kDa
Epitope:
C-terminus
Immunogen:
KLH-conjugated synthetic peptide corresponding to the C-terminal region of human PI3 Kinase, p110α
Isotype:
IgG
Background Information:
PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
Species Reactivity:
Human
Species Reactivity Note:
Proven to react with human. Reactivity with other species has not been confirmed.
Not believed to cross-react with mouse or rat.
Application Notes:
Immunoprecipitation:
A 1:30 dilution of a previous lot immunoprecipitated PI3 Kinase, p110a using protein G agarose beads.
Immunfluorescence Staining:
1:100-1:250 dilution of a previous lot was used for Immunfluorescence staining of paraformaldehyde fixed HeLa cells.
Control:
Jurkat cell lysate
Quality Assurance:
Routinely evaluated by Western Blot on jurkat cell lysate.
Western Blot Analysis:
A 1:5,000 dilution of the lot detected endogenous PI3 Kinase, p110α in lysate from Jurkat cells.
Purification Method:
Purified
Presentation:
Purified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide, 0.05% BSA and 40% glycerol.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
UniProt Number:
P42336
Entrez Gene Number:
NM_006218.2
Gene Symbol:
PIK3CA
MGC142161
PI3K
p110-alpha
MGC142163
Alternate Names:
PI3-kinase p110 subunit alpha
PtdIns-3-kinase p110
phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha
phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit,
alpha isoform
phosphoinositide-3-kinase, catalytic, alpha polypeptide
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses |