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Anti-MEK1 CT Antibody, rabbit monoclonal
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REFERENCES
Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway.
Drosopoulos, Konstantinos G, et al. (2005) J. Biol. Chem., 280: 22856-67 (2005)
Activation of the NaCl- and drought-induced RD29A and RD29B promoters by constitutively active Arabidopsis MAPKK or MAPK proteins
Hua, Zhi-Ming, et al (2006) Plant Cell Environ, 29:1761-70 (2006)
p38 Mitogen-activated protein kinase pathway suppresses cell survival by inducing dephosphorylation of mitogen-activated protein/extracellular signal-regulated kinase kinase1,2
Li, Song-Ping, et al (2003) Cancer Res, 63:3473-7 (2003)
Species Reactivity Key Applications Host Format Antibody Type
H, R FC, ICC, IH(P), IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-MEK1 CT Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes the C-terminal domain of MEKK1.
Molecular Weight:
45 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to the C-terminal region of human MEK1.
Isotype:
IgG
Species Reactivity:
Human
Rat
Application Notes:
Suggested dilutions:
Immunoblot Analysis: 1:10,000
Immunoprecipitation: 1:150
Flow Cytometry: 1:40
Immunohistochemistry: A 1:250
Immunfluorescence Staining: 1:250-1:500
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Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
In 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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UniProt Number:
Q02750
Entrez Gene Number:
NM_002755.2
Gene Symbol:
MAP2K1
PRKMK1
MKK1
MAPKK1
MEK1
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Alternate Names:
MAPKK1, MAP2K1, MKK1, PRKMK1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
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Entrez Gene Summary:
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.< |