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Anti-AMPKα1 Antibody, rabbit monoclonal
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REFERENCES
TSC2 integrates Wnt and energy signals via a coordinated phosphorylation by AMPK and GSK3 to regulate cell growth.
Inoki, Ken, et al. (2006) Cell, 126: 955-68 (2006)
AMP-activated protein kinase suppresses protein synthesis in rat skeletal muscle through down-regulated mammalian target of rapamycin (mTOR) signaling
Bolster, Douglas R, et al (2002) J Biol Chem, 277:23977-80 (2002)
Glucose metabolism and cancer
Shaw, Reuben J (2006) Curr Opin Cell Biol, 18:598-608 (2006)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R IH(P), IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-AMPKα1 Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes the C-terminal domain of AMPKα1.
Molecular Weight:
63 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to the C-terminal region of human AMPKα1.
Isotype:
IgG
Species Reactivity:
Human
Mouse
Rat
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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UniProt Number:
Q13131
Entrez Gene Number:
NM_006251.5
NM_206907.3
Gene Symbol:
PRKAA1
MGC57364
AMPK
AMPK1
MGC33776
AMPKa1
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Alternate Names:
PRKAA1, protein kinase, AMP-activated, alpha 1 catalytic subunit, AMPK, AMPKa1, AMPK alpha 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
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UniProt Summary:
FUNCTION: SwissProt: Q13131 # Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathw |