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Anti-4E-BP1 Antibody, rabbit monoclonal
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REFERENCES
The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif
Nojima, Hiroki, et al (2003) J Biol Chem, 278:15461-4 (2003)
eIF4E binding protein 1 and H-Ras are novel substrates for the protein kinase activity of class-I phosphoinositide 3-kinase
Foukas, Lazaros C and Shepherd, Peter R (2004) Biochem Biophys Res Commun, 319:541-9 (2004)
Insulin activation of Rheb, a mediator of mTOR/S6K/4E-BP signaling, is inhibited by TSC1 and 2
Garami, Attila, et al (2003) Mol Cell, 11:1457-66 (2003)
Species Reactivity Key Applications Host Format Antibody Type
H FC, WB, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-4E-BP1 Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes the C-terminal domain of 4E-BP1.
Molecular Weight:
15-20 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to the C-terminal region of human 4E-BP1 (eIF4E-binding protein).
Isotype:
IgG
Species Reactivity:
Human
Species Reactivity Note:
Not expected to react with mouse or rat.
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
100 μL of rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 0.09 M NaCl, 0.006% sodium azide and 0.03% BSA and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment
UniProt Number:
Q13541
Entrez Gene Number:
NM_004095.3
Gene Symbol:
EIF4EBP1
PHAS-I
4EBP1
MGC4316
4E-BP1
BP-1
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Alternate Names:
EIF4EBP1, eIF4E-binding protein 1, eukaryotic translation initiation factor 4E binding protein 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Western Blotting
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
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UniProt Summary:
FUNCTION: SwissProt: Q13541 # Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway.
SIZE: 118 amino acids; 12580 Da
SUBUNIT: Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.
PTM: Phosphorylated on serine and threon |